Anticorps Polyclonal de lapin anti-ACK1

• Phare
• Validé par KD/KO

ACK1 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 14304-1-AP

Synonymes

ACK, ACK 1, ACK1, Activated CDC42 kinase 1, p21cdc42Hs, TNK2

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Galerie de données de validation

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  WB analysis of A549 using 14304-1-AP

  WB result of ACK1 antibody (14304-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACK1 transfected A549 cells.

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  WB analysis of SH-SY5Y using 14304-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14304-1-AP (ACK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 14304-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 14304-1-AP (ACK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 14304-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 14304-1-AP (ACK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14304-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14304-1-AP (ACK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14304-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14304-1-AP (ACK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14304-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14304-1-AP (ACK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 14304-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14304-1-AP (ACK1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 14304-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14304-1-AP (ACK1 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, cellules A431, cellules A549, cellules HeLa, cellules HepG2, cellules HT-1080, tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

14304-1-AP cible ACK1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ACK1 Protéine recombinante Ag5419
• Nom complet: tyrosine kinase, non-receptor, 2
• Masse moléculaire calculée: 115 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC028164
• Symbole du gène: TNK2
• Identification du gène (NCBI): 10188
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TNK2, also named ACK1, belongs to the protein kinase superfamily and Tyr protein kinase family. It is a non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, and cell proliferation. TNK2 transduces extracellular signals to cytosolic and nuclear effectors. It is a downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. TNK2 confers metastatic properties on cancer cells by negatively regulating tumor suppressors such as WWOX and positively regulating pro-survival factors such as AKT1 and AR. The 60-70kd band is the isoform 2 of TNK2. Isoform 1 and 3 is about 120-125kd.

Publications

Species	Application	Title
human	WB	Cell Microbiol; The Legionella pneumophila effector WipA disrupts host F-actin polymerisation by hijacking phosphotyrosine signalling.; Authors - Lei He
mouse	WB	Biochim Biophys Acta Mol Cell Res; Hsc70 phosphorylation patterns and calmodulin regulate AP2 CCV life span for cell adhesion protein transport; Authors - G F Sengül