Anticorps Polyclonal de lapin anti-TNKS

• Phare
• Validé par KD/KO

TNKS Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(11)

Avis (1)

N° de cat : 18030-1-AP

Synonymes

PARP5A, PARP 5a, EC:2.4.2.30, EC:2.4.2.-, ARTD5

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Galerie de données de validation

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  WB analysis of HeLa using 18030-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18030-1-AP (TNKS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 18030-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18030-1-AP (TNKS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 18030-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 18030-1-AP (TNKS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 18030-1-AP

  IP result of anti-TNKS (IP:18030-1-AP, 4ug; Detection:18030-1-AP 1:500) with Jurkat cells lysate 3200ug.

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  IHC staining of mouse testis using 18030-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18030-1-AP (TNKS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 18030-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18030-1-AP (TNKS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 18030-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18030-1-AP (TNKS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 18030-1-AP

  Immunofluorescent analysis of HepG2 cells, using TNKS antibody 18030-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules MCF-7
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu testiculaire de souris, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

18030-1-AP cible TNKS dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TNKS Protéine recombinante Ag12597
• Nom complet: tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase
• Masse moléculaire calculée: 1327 aa, 142 kDa
• Poids moléculaire observé: 150 kDa
• Numéro d’acquisition GenBank: BC098394
• Symbole du gène: TNKS
• Identification du gène (NCBI): 8658
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
	WB	Mol Cell; PARP5A and RNF146 phase separation restrains RIPK1-dependent necroptosis; Authors - Shouqiao Hou
		J Biol Chem; The dePARylase NUDT16 promotes radiation resistance of cancer cells by blocking SETD3 for degradation via reversing its ADP-ribosylation; Authors - Weijun Wu
Mouse		J Cell Sci; Knockout of Syntaxin-4 in 3T3-L1 adipocytes reveals new insight into GLUT4 trafficking and adiponectin secretion.; Authors - Hannah L Black
human	IHC, WB, IF	Oncol Lett; Dihydroartemisinin suppresses proliferation, migration, the Wnt/β-catenin pathway and EMT via TNKS in gastric cancer.; Authors - Yanmei Ma
human	WB,IHC	Cell Death Dis; circ5615 functions as a ceRNA to promote colorectal cancer progression by upregulating TNKS.; Authors - Zhifei Ma ; KD Validated
human	WB	Adv Sci (Weinh); Transferrin Receptor-Mediated Iron Uptake Promotes Colon Tumorigenesis; Authors - Hyeoncheol Kim

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aditya (Verified Customer) (06-16-2025)

working with a hek lysate western blot, did not get any banding in the predicted protein range specified by proteintech - cut the blot to only have this region, may have run over or under and i wouldnt be able to tell

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

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