Anticorps Polyclonal de lapin anti-TNNC1

• Phare
• Validé par KD/KO

TNNC1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13504-1-PBS

Synonymes

TNNC, TN-C, TNC, TN C

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Galerie de données de validation

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  WB analysis of mouse skeletal muscle using 13504-1-AP (same clone as 13504-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13504-1-AP (TNNC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13504-1-PBS in a different storage buffer formulation.

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  WB analysis of human stomach using 13504-1-AP (same clone as 13504-1-PBS)

  human stomach tissue were subjected to SDS PAGE followed by western blot with 13504-1-AP (TNNC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13504-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse heart using 13504-1-AP (same clone as 13504-1-PBS)

  IP result of anti-TNNC1 (IP:13504-1-AP, 4ug; Detection:13504-1-AP 1:600) with mouse heart tissue lysate 4400ug. This data was developed using the same antibody clone with 13504-1-PBS in a different storage buffer formulation.

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  IHC staining of human skeletal muscle using 13504-1-AP (same clone as 13504-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 13504-1-AP (TNNC1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 13504-1-PBS in a different storage buffer formulation.

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  IHC staining of human skeletal muscle using 13504-1-AP (same clone as 13504-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 13504-1-AP (TNNC1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 13504-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 13504-1-AP (same clone as 13504-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13504-1-AP (TNNC1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13504-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 13504-1-AP (same clone as 13504-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13504-1-AP (TNNC1 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13504-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 13504-1-AP (same clone as 13504-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using TNNC1 antibody (13504-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13504-1-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 13504-1-AP (same clone as 13504-1-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.4 ug TNNC1 Polyclonal antibody (13504-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 13504-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13504-1-PBS cible TNNC1 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TNNC1 Protéine recombinante Ag4339
• Nom complet: troponin C type 1 (slow)
• Masse moléculaire calculée: 161 aa, 18 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC030244
• Symbole du gène: TNNC1
• Identification du gène (NCBI): 7134
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Troponin is a central regulatory protein of striated muscle contraction, and together with tropomyosin, is located on the actin filament. Troponin consists of 3 subunits: TnI, which is the inhibitor of actomyosin ATPase; TnT, which contains the binding site for tropomyosin; and TnC, the protein encoded by this gene. TnC plays an important role in excitation-contraction coupling and is the primary target of compounds such as levosimendan that augment Ca2+ responsiveness of the thin filament.

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