Anticorps Polyclonal de lapin anti-TNP1
TNP1 Polyclonal Antibody for IF, WB,ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris
Applications
WB, IHC, IF, ELISA
Conjugaison
Non conjugué
N° de cat : 17178-1-AP
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | tissu testiculaire de souris |
| Résultats positifs en IF | tissu testiculaire de souris, |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| WB | See 8 publications below |
| IHC | See 3 publications below |
| IF | See 7 publications below |
Informations sur le produit
17178-1-AP cible TNP1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
| Réactivité | Humain, rat, souris |
| Réactivité citée | Humain, souris |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | TNP1 Protéine recombinante Ag10890 |
| Nom complet | transition protein 1 (during histone to protamine replacement) |
| Masse moléculaire calculée | 55 aa, 7 kDa |
| Poids moléculaire observé | 6-10 kDa |
| Numéro d’acquisition GenBank | BC029516 |
| Symbole du gène | TNP1 |
| Identification du gène (NCBI) | 7141 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par affinité contre l'antigène |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Transition nuclear proteins (TNP1 and TNP2) are the major nuclear proteins that replace somatic histones during spermatogenesis. TNPs are required for normal chromatin condensation and functional sperm development, spermatogenesis was found to be compromised in both Tnp1 and Tnp2 null mice. TNP1, or TP1, localized in nucleus, is a spermatid-specific product of the haploid genome which replaces histone and is itself replaced in the mature sperm by the protamines. Recently, TNP-1 was used as a germ cell marker in condensing spermatids.
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for TNP1 antibody 17178-1-AP | Download protocol |
| IF protocol for TNP1 antibody 17178-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Ubiquitination-Deficient Mutations in Human Piwi Cause Male Infertility by Impairing Histone-to-Protamine Exchange during Spermiogenesis. | ||
Mol Cell Chromodomain Protein CDYL Acts as a Crotonyl-CoA Hydratase to Regulate Histone Crotonylation and Spermatogenesis. | ||
J Hazard Mater Multi-omics analyses reveal the mechanisms of Arsenic-induced male reproductive toxicity in mice. | ||
Elife Absence of CEP78 causes photoreceptor and sperm flagella impairments in mice and a human individual | ||
Environ Pollut Silica nanoparticles induce spermatogenesis disorders via L3MBTL2-DNA damage-p53 apoptosis and RNF8-ubH2A/ubH2B pathway in mice. | ||
Environ Pollut Silica nanoparticles inhibiting the differentiation of round spermatid and chromatin remodeling of haploid period via MIWI in mice. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Chongil (Verified Customer) (08-19-2021) | This is a great IF antibody to detect Tnp1 protein.
 |
FH Zhenminm (Verified Customer) (07-08-2019) | This antibody is highly specific and works excellently for immunohistochemistry as well as immunofluorescence in formalin fixed and paraffin embedded tissue sections.
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