Anticorps Polyclonal de lapin anti-TNP1

TNP1 Polyclonal Antibody for WB, IF-P, IF-Fro, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF-P, IF-Fro, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 17178-1-PBS

Synonymes

STP-1, TP-1

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Galerie de données de validation

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  WB analysis of mouse testis using 17178-1-AP (same clone as 17178-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17178-1-AP (TNP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17178-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 17178-1-AP (same clone as 17178-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17178-1-AP (TNP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17178-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 17178-1-AP (same clone as 17178-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 17178-1-AP (TNP1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17178-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 17178-1-AP (same clone as 17178-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse testis tissue using 17178-1-AP (TNP1 antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17178-1-PBS in a different storage buffer formulation.

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Informations sur le produit

17178-1-PBS cible TNP1 dans les applications de WB, IF-P, IF-Fro, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TNP1 Protéine recombinante Ag10890
• Nom complet: transition protein 1 (during histone to protamine replacement)
• Masse moléculaire calculée: 55 aa, 7 kDa
• Poids moléculaire observé: 6-10 kDa
• Numéro d’acquisition GenBank: BC029516
• Symbole du gène: TNP1
• Identification du gène (NCBI): 7141
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Transition nuclear proteins (TNP1 and TNP2) are the major nuclear proteins that replace somatic histones during spermatogenesis. TNPs are required for normal chromatin condensation and functional sperm development, spermatogenesis was found to be compromised in both Tnp1 and Tnp2 null mice. TNP1, or TP1, localized in nucleus, is a spermatid-specific product of the haploid genome which replaces histone and is itself replaced in the mature sperm by the protamines. Recently, TNP-1 was used as a germ cell marker in condensing spermatids.

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