Anticorps Recombinant de lapin anti-TNPO3

TNPO3 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240728F8

Avis (1)

N° de cat : 83704-2-RR

Synonymes

transportin 3, IPO12, Importin-12, Importin 12, Imp12

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Galerie de données de validation

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  WB analysis using 83704-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83704-2-RR (TNPO3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 83704-2-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug TNPO3 Recombinant antibody (83704-2-RR, Clone:240728F8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  FC experiment of U-2 OS using 83704-2-RR

  1x10^6 U-2 OS were intracellularly stained with 0.25 ug TNPO3 Recombinant antibody (83704-2-RR, Clone:240728F8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 83704-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83704-2-RR against Human TNPO3 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules C2C12, cellules NIH/3T3, tissu cérébral de souris, tissu de thymus de rat
• Résultats positifs en FC (Intra): cellules HeLa, cellules U2OS

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83704-2-RR cible TNPO3 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TNPO3 Protéine recombinante Ag8769
• Nom complet: transportin 3
• Masse moléculaire calculée: 975 aa, 110 kDa
• Poids moléculaire observé: 104 kDa
• Numéro d’acquisition GenBank: BC009923
• Symbole du gène: TNPO3
• Identification du gène (NCBI): 23534
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Transportin 3 (TNPO3) also known as IPO12, TRNSR, is a karyopherin β that works as a nuclear carrier shuttling, from the cytoplasm to the nucleus, the serine/arginine-rich proteins (PMID: 33452620). TNPO3 specifically mediates the nuclear import of splicing factor serine/arginine (SR) proteins, such as RBM4, SFRS1, and SFRS2, by recognizing phosphorylated SR domains (PMID: 11517331, 12628928). Mutations in TNPO3 are associated with Autosomal Dominant Limb-Girdle Muscular Dystrophy 2 (PMID: 23543484).

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Fabienne (Verified Customer) (09-12-2025)

Works great for Western blot (tested on nuclear extracts)

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Jurkat

TNPO3 Antibody Western Blot validation (1:1000 dilution) in Jurkat (Cat no:83704-2-RR)

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