Anticorps Polyclonal de lapin anti-Tenascin-R

Tenascin-R Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 19730-1-AP

Synonymes

Janusin, Restrictin, Tenascin R, TN R, TNR

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Galerie de données de validation

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  WB analysis of human brain using 19730-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19730-1-AP (Tenascin-R antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 19730-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19730-1-AP (Tenascin-R antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 19730-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 19730-1-AP (Tenascin-R antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 19730-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 19730-1-AP (Tenascin-R antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, cellules SH-SY5Y
• Résultats positifs en IHC: tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

19730-1-AP cible Tenascin-R dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: tenascin R (restrictin, janusin)
• Masse moléculaire calculée: 150 kDa
• Poids moléculaire observé: 180 kDa, 160 kDa
• Numéro d’acquisition GenBank: NM_003285
• Symbole du gène: TNR
• Identification du gène (NCBI): 7143
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TNR, also named as Restrictin and Janusin, belongs to the tenascin family. Neural extracellular matrix (ECM) protein involved in interactions with different cells and matrix components. These interactions can influence cellular behavior by either evoking a stable adhesion and differentiation, or repulsion and inhibition of neurite growth. Binding to cell surface gangliosides, TNR inhibits RGD-dependent integrin-mediated cell adhesion and results in an inhibition of PTK2 (FAK) phosphorylation and cell detachment. Binding to membrane surface sulfatides, TNR results in a oligodendrocyte adhesion and differentiation. Interaction with CNTN1, TNR induces a repulsion of neurons and an inhibition of neurite outgrowth. Interacts with SCN2B, TNR may play a crucial role in clustering and regulation of activity of sodium channels at nodes of Ranvier. TNR-linked chondroitin sulfate glycosaminoglycans are involved in the interaction with FN1 and mediate inhibition of cell adhesion and neurite outgrowth. The highly regulated addition of sulfated carbohydrate structure may modulate the adhesive properties of TNR over the course of development and during synapse maintenance. The antibody is specific to TNR.

Publications

Species	Application	Title
human	WB,IHC	Front Immunol; Immune Gene Signatures and Immunotypes in Immune Microenvironment Are Associated With Glioma Prognose.; Authors - Xiang-Xu Wang