Anticorps Polyclonal de lapin anti-TNS4

• Phare
• Validé par KD/KO

TNS4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 11580-1-AP

Synonymes

Tensin-4, tensin 4, PP14434, C-terminal tensin-like protein, CTEN

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Galerie de données de validation

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  WB analysis using 11580-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11580-1-AP (TNS4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 11580-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11580-1-AP (TNS4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 11580-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11580-1-AP (TNS4 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 11580-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11580-1-AP (TNS4 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of A549 using 11580-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 11580-1-AP (TNS4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules NIH/3T3, cellules PC-12, cellules RAW 264.7, tissu placentaire humain
• Résultats positifs en IHC: tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

11580-1-AP cible TNS4 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TNS4 Protéine recombinante Ag2169
• Nom complet: tensin 4
• Masse moléculaire calculée: 715 aa, 77 kDa
• Poids moléculaire observé: 77 kDa
• Numéro d’acquisition GenBank: BC018706
• Symbole du gène: TNS4
• Identification du gène (NCBI): 84951
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TNS4 (Tensin-4), also known as COOH-terminus tensin-like protein, is a distinctive member with the smallest molecular mass and lacks the N-terminal common region found in other family members. TNS4 plays a crucial role in CRC tumorigenesis, and that suppression of TNS4 would be an effective therapeutic strategy in treating a subset of cetuximab-refractory CRC patients including KRAS activating mutations (PMID: 31409052). TNS4 has been implicated in numerous vital biological processes, including proliferation, migration, cell adhesion, and apoptosis (PMID: 38164166).

Publications

Species	Application	Title
mouse	IF	Front Bioeng Biotechnol; Smarce1 and Tensin 4 Are Putative Modulators of Corneoscleral Stiffness.; Authors - Elizabeth M Boazak
human	WB,IHC	Oncol Lett; Tensin 4 facilitates aerobic glycolysis, migration and invasion of colorectal cancer cells through the β‑catenin/c‑Myc signaling pathway; Authors - Yan Wang ; KD Validated
human	WB	Pathol Res Pract; Tensin4 promotes invasion and migration of gastric cancer cells via regulating AKT/GSK-3β/snail signaling pathway.; Authors - Xiumin Qi ; KD Validated
human	IHC	Front Immunol; Development and functional validation of a disulfidoptosis-related gene prognostic model for lung adenocarcinoma based on bioinformatics and experimental validation; Authors - Tao Shen
human,mouse	WB,IP,IHC	Cancer Cell Int; TNS4 promotes lymph node metastasis of gastric cancer by interacting with integrin Β1 and inducing the activation of fibroblastic reticular cell; Authors - Xiang Zhang ; KD Validated
human	WB	Antioxid Redox Signal; A novel lncRNA FUAT1/TNS4 axis confers chemoresistance by suppressing ROS-mediated apoptosis in gastric cancer; Authors - Mingliang Liu

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