Anticorps Polyclonal de lapin anti-Tenascin-X

Tenascin-X Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

Avis (1)

N° de cat : 13595-1-AP

Synonymes

Hexabrachion like protein, HXBL, Tenascin X, tenascin XB, Tenascin-X, TENX, TN X, TNX, TNXB, TNXB1, TNXB2, TNXBS, XB, XBS

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Galerie de données de validation

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  WB analysis of mouse liver using 13595-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13595-1-AP (Tenascin-X antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 13595-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13595-1-AP (Tenascin-X antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 13595-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 13595-1-AP (Tenascin-X antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 13595-1-AP

  IP Result of anti-Tenascin-X (IP:13595-1-AP, 4ug; Detection:13595-1-AP 1:300) with mouse liver tissue lysate 4000ug.

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  IHC staining of human kidney using 13595-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13595-1-AP (Tenascin-X antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung using 13595-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 13595-1-AP (Tenascin-X antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 13595-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 13595-1-AP (Tenascin-X antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 13595-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 13595-1-AP (Tenascin-X antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 13595-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13595-1-AP (Tenascin-X antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules HT-1080, tissu hépatique de rat
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu rénal humain, tissu cardiaque humain, tissu de cancer du sein humain, tissu hépatique humain, tissu pulmonaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

13595-1-AP cible Tenascin-X dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Tenascin-X Protéine recombinante Ag4501
• Nom complet: tenascin XB
• Masse moléculaire calculée: 4289 aa, 464 kDa
• Poids moléculaire observé: 75 kDa, 140 kDa
• Numéro d’acquisition GenBank: BC033740
• Symbole du gène: TNXB
• Identification du gène (NCBI): 7148
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tenascin-X (TNXB) is an extracellular matrix glycoprotein predominantly located in the outer reticular lamina of the basement membrane (BM) (PMID: 23768946). It interacts with many other ECM proteins and it accelerates collagen fibrillogenesis in vitro. TNXB plays a role in interactions between cell and ECM, antiadhesive effect, inhibiting cell migration, and in maintaining homeostasis of the extracellular matrix. Deficiency of TNXB has been associated with the connective tissue disorder Ehlers-Danlos syndrome. This antibody is raised against human TNXB and detects a band of about 75 kDa, which probably represents a proteolytic cleaved form of human TNXB (PMID: 17263730). But the serum form of tenascin-X is a 140-kD protein probably resulting from proteolytic cleavage or alternative splicing of tenascin-X(PMID: 16567571, 11642233).

Publications

Species	Application	Title
human	WB,IHC,IF	J Am Soc Nephrol; TNXB mutations can cause vesicoureteral reflux.; Authors - Rasheed A Gbadegesin
human	IF	Matrix Biol; Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.; Authors - Emna Ouni
human	WB,IHC	World J Gastroenterol; Role of Tenascin-X in regulating TGF-β/Smad signaling pathway in pathogenesis of slow transit constipation.; Authors - Yi-Chao Zhang
human	WB,IHC	Mol Oncol; Integrated proteome and phosphoproteome analysis of gastric adenocarcinoma reveals molecular signatures capable of stratifying patient outcome; Authors - Xue Lu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Iram (Verified Customer) (09-04-2020)

Very sharp bands of TenascinX in western blotting

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000 for western
• Cell Tissue Type: Human colon cancer