Anticorps Recombinant de lapin anti-TOB1

TOB1 Recombinant Antibody for IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241043A9

N° de cat : 83944-2-PBS

Synonymes

241043A9, APRO6, PIG49, Protein Tob1, TOB

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Galerie de données de validation

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  IHC staining of human stomach cancer using 83944-2-RR (same clone as 83944-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83944-2-RR (TOB1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83944-2-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 83944-2-RR (same clone as 83944-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83944-2-RR (TOB1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83944-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83944-2-RR (same clone as 83944-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TOB1 antibody (83944-2-RR, Clone: 241043A9 ) at dilution of 1:0 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83944-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00930-3

  Cytometric bead array standard curve of MP00930-3, TOB1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83944-1-PBS. Detection antibody: 83944-2-PBS. Standard: Ag6714. Range: 0.313-20 ng/mL.

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  Affinity and Kinetic Characterization of 83944-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83944-2-RR against Human TOB1 were performed. The affinity constant is 0.462 nM.

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Informations sur le produit

83944-2-PBS cible TOB1 dans les applications de IHC, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TOB1 Protéine recombinante Ag6714
• Nom complet: transducer of ERBB2, 1
• Masse moléculaire calculée: 38 kDa
• Numéro d’acquisition GenBank: BC031406
• Symbole du gène: TOB1
• Identification du gène (NCBI): 10140
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TOB1, also known as APRO6 or TROB1, is a member of the transducer of erbB-2/B-cell translocation gene protein family. Members of this family are anti-proliferative factors that have the potential to regulate cell growth. The TOB1 protein has a large additional C-terminal region, which contains PAM2 motifs that mediate interactions with poly(A)-binding proteins. It is mainly located in the cell nucleus and will transfer between the cell nucleus and the cytoplasm. The more phosphorylation, the more obvious the cytoplasmic localization.

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