Anticorps Monoclonal anti-TOE1

TOE1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2C10H7

N° de cat : 67829-1-PBS

Synonymes

FLJ13949, Target of EGR1 protein 1, TOE1

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Galerie de données de validation

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  WB analysis using 67829-1-Ig (same clone as 67829-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67829-1-Ig (TOE1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67829-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67829-1-Ig (same clone as 67829-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67829-1-Ig (TOE1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67829-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67829-1-Ig (same clone as 67829-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67829-1-Ig (TOE1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67829-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67829-1-Ig (same clone as 67829-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67829-1-Ig (TOE1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67829-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67829-1-Ig (same clone as 67829-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67829-1-Ig (TOE1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67829-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67829-1-Ig (same clone as 67829-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TOE1 antibody (67829-1-Ig, Clone: 2C10H7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67829-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67829-1-PBS cible TOE1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TOE1 Protéine recombinante Ag28982
• Nom complet: target of EGR1, member 1 (nuclear)
• Masse moléculaire calculée: 510 aa, 57 kDa
• Poids moléculaire observé: 57 kDa
• Numéro d’acquisition GenBank: BC009364
• Symbole du gène: TOE1
• Identification du gène (NCBI): 114034
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TOE1, also known as hCaf1z, belongs to the DEDD family of deadenylases. TOE1 was initially identified as a direct target gene of ERG1, an immediate early transcription factor. TOE1 encodes an Asp-Glu-Asp-Asp deadenylase that specifically localizes to Cajal bodies in the nucleus, where assembly and maturation of small nuclear ribonucleoprotein particles, and telomerase ribonucleoprotein complex take place (PMID: 36257135). TOE1 has 2 isoforms with the molecular mass of 48 and 57 kDa.

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