Anticorps Monoclonal anti-TOLLIP

• Phare
• Validé par KD/KO

TOLLIP Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

1B3C4

N° de cat : 68170-1-PBS

Synonymes

1B3C4, IL 1RAcPIP, toll interacting protein, Toll-interacting protein

---

Galerie de données de validation

• 

  WB analysis using 68170-1-Ig (same clone as 68170-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68170-1-Ig (TOLLIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68170-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68170-1-Ig (same clone as 68170-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68170-1-Ig (TOLLIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68170-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 68170-1-Ig (same clone as 68170-1-PBS)

  WB result of TOLLIP antibody (68170-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOLLIP transfected HepG2 cells. This data was developed using the same antibody clone with 68170-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 68170-1-Ig (same clone as 68170-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 68170-1-Ig (TOLLIP antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68170-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 68170-1-Ig (same clone as 68170-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 68170-1-Ig (TOLLIP antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68170-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat colon using 68170-1-Ig (same clone as 68170-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 68170-1-Ig (TOLLIP antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68170-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 68170-1-Ig (same clone as 68170-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using TOLLIP antibody (68170-1-Ig, Clone: 1B3C4 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68170-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Jurkat using 68170-1-Ig (same clone as 68170-1-PBS)

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human TOLLIP (68170-1-Ig, Clone:1B3C4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68170-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

68170-1-PBS cible TOLLIP dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TOLLIP Protéine recombinante Ag32731
• Nom complet: toll interacting protein
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC012057
• Symbole du gène: TOLLIP
• Identification du gène (NCBI): 54472
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TOLLIP (Toll interacting protein), is an inhibitory adaptor protein. Tollip is a negative regulator of IL-1R and Toll-like receptors signalling pathway, involved in inflammatory immune response and inhibiting IRAK1 phosphorylation and kinase activity (PMID:11441107, PMID:10854325, PMID:11751856). The TOLLIP-dependent selective autophagy pathway plays a role in clearance of cytotoxic polyQ proteins aggregates (PubMed:25042851).

{{ptg:RelatedPrimaryAntibodies}}