Anticorps Recombinant de lapin anti-TOM20

TOM20 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, poisson-zèbre, porc, poulet, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D22

N° de cat : 80501-1-PBS

Synonymes

TOMM20, 1D22, KIAA0016, MAS20, MOM19

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Galerie de données de validation

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  WB analysis using 80501-1-RR (same clone as 80501-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80501-1-RR (TOM20 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80501-1-PBS in a different storage buffer formulation.

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  WB analysis of zebrafish using 80501-1-RR (same clone as 80501-1-PBS)

  zebrafish tissue were subjected to SDS PAGE followed by western blot with 80501-1-RR (TOM20 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80501-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 80501-1-RR (same clone as 80501-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80501-1-RR (TOM20 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80501-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 80501-1-RR (same clone as 80501-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80501-1-RR (TOM20 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80501-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 80501-1-RR (same clone as 80501-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TOM20 antibody (80501-1-RR, Clone: 1D22 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80501-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 80501-1-RR (same clone as 80501-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TOM20 antibody (80501-1-RR, Clone: 1D22 ) at dilution of 1:240 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80501-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 80501-1-RR (same clone as 80501-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using TOM20 antibody (80501-1-RR, Clone: 1D22 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 80501-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 80501-1-RR (same clone as 80501-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TOM20 antibody (80501-1-RR, Clone: 1D22 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 80501-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 80501-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80501-1-RR against Human TOM20 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

80501-1-PBS cible TOM20 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, poisson-zèbre, porc, poulet, rat, souris

• Réactivité: Humain, poisson-zèbre, porc, poulet, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TOM20 Protéine recombinante Ag2378
• Nom complet: translocase of outer mitochondrial membrane 20 homolog (yeast)
• Masse moléculaire calculée: 145 aa, 16 kDa
• Poids moléculaire observé: 16 kDa
• Numéro d’acquisition GenBank: BC000882
• Symbole du gène: TOM20
• Identification du gène (NCBI): 9804
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TOM20, also named as KIAA0016, belongs to the Tom20 family. It is a central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22, TOM20 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. TOM20 is characterized as major docking receptors to mediate the recognition by different mechanisms.

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