Anticorps Monoclonal anti-TOM70

• Phare
• Validé par KD/KO

TOM70 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1H8A1

N° de cat : 66593-1-PBS

Synonymes

TOMM70A, Translocase of outer mitochondrial membrane protein 70, TOMM70, TOMM, KIAA0719

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Galerie de données de validation

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  WB analysis using 66593-1-Ig (same clone as 66593-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66593-1-Ig (TOM70 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66593-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66593-1-Ig (same clone as 66593-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66593-1-Ig (TOM70 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66593-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66593-1-Ig (same clone as 66593-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66593-1-Ig (TOM70 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66593-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 66593-1-Ig (same clone as 66593-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66593-1-Ig (TOM70 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66593-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66593-1-Ig (same clone as 66593-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66593-1-Ig (TOM70 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66593-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66593-1-Ig (same clone as 66593-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66593-1-Ig (TOM70 antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66593-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66593-1-PBS cible TOM70 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TOM70 Protéine recombinante Ag6027
• Nom complet: translocase of outer mitochondrial membrane 70 homolog A (S. cerevisiae)
• Masse moléculaire calculée: 67 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC052994
• Symbole du gène: TOM70
• Identification du gène (NCBI): 9868
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TOM70 is a mitochondrial import receptor responsible for the import of cytosol synthesized precursor proteins into the mitochondria. Tom70 is a member of the tetratricopeptide repeat (TPR) co-chaperone family and contains a conserved TPR clamp domain for interaction with Hsc70 and Hsp90. Recently overexpression of TOM70 has been linked to chemoresistance to drugs in breast cancer cell lines. It is also believed that Tom70 is significantly associated with breast cancer relapse and may predict the recurrence of ovarian cancer.

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