Anticorps Monoclonal anti-TOMM40

• Phare
• Validé par KD/KO

TOMM40 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

5E2C5

N° de cat : 66658-1-PBS

Synonymes

TOM40, 5E2C5, C19orf1, D19S1177E, p38.5

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Galerie de données de validation

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  WB analysis of HeLa using 66658-1-Ig (same clone as 66658-1-PBS)

  WB result of TOMM40 antibody (66658-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOMM40 transfected HeLa cells. This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.

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  WB analysis using 66658-1-Ig (same clone as 66658-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66658-1-Ig (TOMM40 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66658-1-Ig (same clone as 66658-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66658-1-Ig (TOMM40 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66658-1-Ig (same clone as 66658-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66658-1-Ig (TOMM40 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 66658-1-Ig (same clone as 66658-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66658-1-Ig (TOMM40 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 66658-1-Ig (same clone as 66658-1-PBS)

  pig brain tissue, rat brain tissue, and mouse brain tissue were subjected to SDS PAGE followed by western blot with 66658-1-Ig (TOMM40 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66658-1-Ig (same clone as 66658-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66658-1-Ig (TOMM40 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 66658-1-Ig (same clone as 66658-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66658-1-Ig (TOMM40 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66658-1-Ig (same clone as 66658-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66658-1-Ig (TOMM40 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66658-1-Ig (same clone as 66658-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66658-1-Ig (TOMM40 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66658-1-Ig (same clone as 66658-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66658-1-Ig (TOMM40 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66658-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66658-1-PBS cible TOMM40 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TOMM40 Protéine recombinante Ag13065
• Nom complet: translocase of outer mitochondrial membrane 40 homolog (yeast)
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC017224
• Symbole du gène: TOMM40
• Identification du gène (NCBI): 10452
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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