Anticorps Recombinant de lapin anti-TOP2A

TOP2A Recombinant Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1J18

N° de cat : 82738-4-PBS

Synonymes

1J18, DNA topoisomerase 2 alpha, DNA topoisomerase 2-alpha, EC:5.6.2.2, TOP II

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Galerie de données de validation

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  WB analysis using 82738-4-RR (same clone as 82738-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82738-4-RR (TOP2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82738-4-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 82738-4-RR (same clone as 82738-4-PBS)

  IP result of anti-TOP2A (IP:82738-4-RR, 4ug; Detection:82738-4-RR 1:2000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 82738-4-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 82738-4-RR (same clone as 82738-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using TOP2A antibody (82738-4-RR, Clone: 1J18 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594-Phalloidin (red). This data was developed using the same antibody clone with 82738-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82738-4-RR

  Biolayer interferometry (BLl) kinetic assays of 82738-4-RR against Human TOP2A were performed. The affinity constant is 0.928 nM.

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Informations sur le produit

82738-4-PBS cible TOP2A dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TOP2A Protéine recombinante Ag19551
• Nom complet: topoisomerase (DNA) II alpha 170kDa
• Masse moléculaire calculée: 1531 aa, 174 kDa
• Poids moléculaire observé: ~170 kDa
• Numéro d’acquisition GenBank: BC140791
• Symbole du gène: TOP2A
• Identification du gène (NCBI): 7153
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TOP2A, also named as TOP2, belongs to the type II topoisomerase family. Topoisomerase II makes double-strand breaks. TOP2 control the topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2A may play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. DNA topoisomerase IIa (170 kDa, TOP2a/170) is essential in proliferating cells by resolving DNA topological entanglements during chromosome condensation, replication, and segregation.

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