Anticorps Polyclonal de lapin anti-TOP2B

• Phare
• Validé par KD/KO

TOP2B Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20549-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HeLa using 20549-1-AP (same clone as 20549-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 20549-1-AP (TOP2B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20549-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 20549-1-AP (same clone as 20549-1-PBS)

  WB result of TOP2B antibody (20549-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOP2B transfected HepG2 cells. This data was developed using the same antibody clone with 20549-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 20549-1-AP (same clone as 20549-1-PBS)

  WB result of TOP2B antibody (20549-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOP2B transfected HeLa cells. This data was developed using the same antibody clone with 20549-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 20549-1-AP (same clone as 20549-1-PBS)

  WB result of TOP2B antibody (20549-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOP2B transfected HepG2 cells. This data was developed using the same antibody clone with 20549-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 20549-1-AP (same clone as 20549-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 20549-1-AP (TOP2B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20549-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 20549-1-AP (same clone as 20549-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20549-1-AP (TOP2B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20549-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 20549-1-AP (same clone as 20549-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20549-1-AP (TOP2B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20549-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 20549-1-AP (same clone as 20549-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 20549-1-AP (TOP2B antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20549-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 20549-1-AP (same clone as 20549-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 20549-1-AP (TOP2B antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20549-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HepG2 using 20549-1-AP (same clone as 20549-1-PBS)

  IP result of anti-TOP2B (IP:20549-1-AP, 4ug; Detection:20549-1-AP 1:300) with HepG2 cells lysate 3200ug. This data was developed using the same antibody clone with 20549-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 20549-1-AP (same clone as 20549-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20549-1-AP (TOP2B antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20549-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 20549-1-AP (same clone as 20549-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20549-1-AP (TOP2B antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20549-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 20549-1-AP (same clone as 20549-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20549-1-AP (TOP2B antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20549-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 20549-1-AP (same clone as 20549-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20549-1-AP (TOP2B antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20549-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 20549-1-AP (same clone as 20549-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TOP2B antibody (20549-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20549-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 20549-1-AP (same clone as 20549-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using TOP2B antibody 20549-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 20549-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20549-1-PBS cible TOP2B dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: topoisomerase (DNA) II beta 180kDa
• Masse moléculaire calculée: 183 kDa
• Poids moléculaire observé: 183 kDa
• Numéro d’acquisition GenBank: NM_001068
• Symbole du gène: TOP2B
• Identification du gène (NCBI): 7155
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TOP2B belongs to the type II topoisomerase family. TOP2B control topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2B makes double-strand breaks. It is indirectly ivolved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. The antibody is specific to TOP2B.

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