Anticorps Recombinant de lapin anti-TOP2B

• Phare
• Validé par KD/KO

TOP2B Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240949F10

N° de cat : 84049-1-PBS

Synonymes

240949F10, DNA topoisomerase 2-beta, EC:5.6.2.2, top2beta, TOPIIB

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Galerie de données de validation

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  WB analysis using 84049-1-RR (same clone as 84049-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84049-1-RR (TOP2B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84049-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 84049-1-RR (same clone as 84049-1-PBS)

  WB result of TOP2B antibody (84049-1-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOP2B transfected HepG2 cells. This data was developed using the same antibody clone with 84049-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84049-1-RR (same clone as 84049-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TOP2B antibody (84049-1-RR, Clone: 240949F10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84049-1-PBS in a different storage buffer formulation.

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Informations sur le produit

84049-1-PBS cible TOP2B dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: topoisomerase (DNA) II beta 180kDa
• Masse moléculaire calculée: 183 kDa
• Poids moléculaire observé: 180-190 kDa
• Numéro d’acquisition GenBank: NM_001068
• Symbole du gène: TOP2B
• Identification du gène (NCBI): 7155
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TOP2B belongs to the type II topoisomerase family. TOP2B control topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2B makes double-strand breaks. It is indirectly ivolved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.

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