Anticorps Polyclonal de lapin anti-TOP3A

• Phare
• Validé par KD/KO

TOP3A Polyclonal Antibody for IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

25

Avis (1)

N° de cat : 14525-1-AP

Synonymes

DNA topoisomerase 3 alpha, DNA topoisomerase III alpha, TOP3, TOP3A, topoisomerase (DNA) III alpha

---

Galerie de données de validation

• 

  WB analysis of K-562 using 14525-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14525-1-AP (TOP3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 14525-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14525-1-AP (TOP3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 14525-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 14525-1-AP (TOP3A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 14525-1-AP

  IP Result of anti-TOP3A (IP:14525-1-AP, 4ug; Detection:14525-1-AP 1:500) with K-562 cells lysate 3600ug.

---

Applications testées

• Résultats positifs en WB: cellules K-562, cellules HL-60
• Résultats positifs en IP: cellules K-562

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 22 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

14525-1-AP cible TOP3A dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris, xénope
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TOP3A Protéine recombinante Ag6010
• Nom complet: topoisomerase (DNA) III alpha
• Masse moléculaire calculée: 1001 aa, 112 kDa
• Poids moléculaire observé: 100-110 kDa
• Numéro d’acquisition GenBank: BC051748
• Symbole du gène: TOP3A
• Identification du gène (NCBI): 7156
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DNA topoisomerase 3-alpha (TOP3A) is an essential component of the RMI complex, a complex involves in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. It releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. This antibody is specific to react with the 110kd human TOP3A.

Publications

Species	Application	Title
human	WB	Mol Cell; TopBP1 interacts with BLM to maintain genome stability but is dispensable for preventing BLM degradation.; Authors - Andrew N Blackford
human	WB	Mol Cell; Topoisomerase 3α Is Required for Decatenation and Segregation of Human mtDNA.; Authors - Thomas J Nicholls ; KD Validated
		Sci Adv; The mitochondrial single-stranded DNA binding protein is essential for initiation of mtDNA replication.; Authors - Min Jiang
human	WB	Nat Commun; The Bloom syndrome complex senses RPA-coated single-stranded DNA to restart stalled replication forks.; Authors - Ann-Marie K Shorrocks
human	WB	Nucleic Acids Res; PMID: 30169847; Authors - Anu Hangas
human	WB,IHC	Nucleic Acids Res; Top3α is the replicative topoisomerase in mitochondrial DNA replication.; Authors - Anu Hangas ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Andrea (Verified Customer) (01-23-2023)

Strong and crisp band.

• Applications: Western Blot