Anticorps Polyclonal de lapin anti-TOPBP1

TOPBP1 Polyclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 23340-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HeLa using 23340-1-AP (same clone as 23340-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 23340-1-AP (TOPBP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23340-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 23340-1-AP (same clone as 23340-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 23340-1-AP (TOPBP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23340-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 23340-1-AP (same clone as 23340-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 23340-1-AP (TOPBP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23340-1-PBS in a different storage buffer formulation.

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Informations sur le produit

23340-1-PBS cible TOPBP1 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TOPBP1 Protéine recombinante Ag18961
• Nom complet: topoisomerase (DNA) II binding protein 1
• Masse moléculaire calculée: 1522 aa, 171 kDa
• Poids moléculaire observé: 171 kDa
• Numéro d’acquisition GenBank: BC126209
• Symbole du gène: TOPBP1
• Identification du gène (NCBI): 11073
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Human DNA topoisomerase II binding protein 1 (TopBP1) contains eight BRCT motifs that are found in proteins regulating the DNA damage response, transcription, and replication (1). In addition, TopBP1 shares sequence similarity with the fission yeast Rad4/Cut5 protein and the budding yeast DPB11 protein, both of which are required for DNA damage control and/or replication checkpoint control (2,3). Phosphorylation of TopBP1 occurs in response to DNA double-strand breaks and replication blocks (2). TopBP1 forms nuclear foci and localizes to the sites of DNA damage or the arrested replication forks (2,3). Downregulation of TopBP1 leads to reduced cell survival, probably due to increased apoptosis (2). TopBP1 functions as a transcriptional coactivator by enhancing the human papillomavirus (HPV) transcription/replication factor E2 (1). In addition, the HECT-domain ubiquitin ligase, hHYD, cooperates with TopBP1 in DNA damage response (4). TopBP1 specifically interacts with the C-terminal region of topoisomerase II beta, which suggests a supportive role for TopBP1 in the catalytic reactions of topoisomerase II beta through transient breakages of DNA strands (5). The gene encoding TopBP1 maps to chromosome 3q22.2 (5).

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