Anticorps Recombinant de lapin anti-TOX2

TOX2 Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241645A11

N° de cat : 84304-2-PBS

Synonymes

241645A11, C20orf100, GCX1, GCX-1

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Galerie de données de validation

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  IF Staining of A549 using 84304-2-RR (same clone as 84304-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using TOX2 antibody (84304-2-RR, Clone: 241645A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84304-2-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 84304-2-RR (same clone as 84304-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using TOX2 antibody (84304-2-RR, Clone: 241645A11 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green). This data was developed using the same antibody clone with 84304-2-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 84304-2-RR (same clone as 84304-2-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug Tox2 Recombinant Antibody (84304-2-RR, Clone:241645A11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84304-2-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 84304-2-RR (same clone as 84304-2-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Tox2 Recombinant Antibody (84304-2-RR, Clone:241645A11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84304-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01188-1

  Cytometric bead array standard curve of MP01188-1, TOX2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84304-2-PBS. Detection antibody: 84304-1-PBS. Standard: Ag14772. Range: 0.156-20 ng/mL.

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  Cytometric bead array standard curve of MP01188-2

  Cytometric bead array standard curve of MP01188-2, TOX2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84304-2-PBS. Detection antibody: 84304-4-PBS. Standard: Ag14772. Range: 0.156-20 ng/mL.

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  Cytometric bead array standard curve of MP01188-3

  Cytometric bead array standard curve of MP01188-3, TOX2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84304-2-PBS. Detection antibody: 84304-3-PBS. Standard: Ag14772. Range: 0.156-20 ng/mL.

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  Affinity and Kinetic Characterization of 84304-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84304-2-RR against Human TOX2 were performed. The affinity constant is 0.240 nM.

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Informations sur le produit

84304-2-PBS cible TOX2 dans les applications de IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TOX2 Protéine recombinante Ag14772
• Nom complet: TOX high mobility group box family member 2
• Masse moléculaire calculée: 488 aa, 52 kDa
• Numéro d’acquisition GenBank: BC007636
• Symbole du gène: TOX2
• Identification du gène (NCBI): 84969
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TOX2 is a transcription factor belonging to the TOX (thymocyte selection-associated HMG box) family that shares a highly conserved high mobility group DNA-binding domain with the other TOX members. A previous study showed that TOX2 is preferentially expressed in human NK cells among various leukocyte populations and is required for in vitro and in vivo human NK cell differentiation from UCB-derived CD341 hematopoietic stem cells (HSCs) (PMID: 25352127).

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