Anticorps Recombinant de lapin anti-TOX2

TOX2 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241645C11

N° de cat : 84304-5-RR

Synonymes

GCX-1, GCX1, C20orf100, 241645C11

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Galerie de données de validation

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  WB analysis using 84304-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84304-5-RR (TOX2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of A549 using 84304-5-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug TOX2 Recombinant antibody (84304-5-RR, Clone:241645C11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  FC experiment of MCF-7 using 84304-5-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug TOX2 Recombinant antibody (84304-5-RR, Clone:241645C11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 84304-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84304-5-RR against Human TOX2 were performed. The affinity constant is 110 nM.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules HeLa, cellules HepG2, cellules MCF-7, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en FC (Intra): cellules A549, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84304-5-RR cible TOX2 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TOX2 Protéine recombinante Ag14772
• Nom complet: TOX high mobility group box family member 2
• Masse moléculaire calculée: 488 aa, 52 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC007636
• Symbole du gène: TOX2
• Identification du gène (NCBI): 84969
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TOX2 is a transcription factor belonging to the TOX (thymocyte selection-associated HMG box) family that shares a highly conserved high mobility group DNA-binding domain with the other TOX members. A previous study showed that TOX2 is preferentially expressed in human NK cells among various leukocyte populations and is required for in vitro and in vivo human NK cell differentiation from UCB-derived CD341 hematopoietic stem cells (HSCs) (PMID: 25352127).

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