Anticorps Polyclonal de lapin anti-P53

• Phare
• Validé par KD/KO

P53 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat et plus (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

38

Avis (1)

N° de cat : 21891-1-AP

Synonymes

Antigen NY CO 13, Cellular tumor antigen p53, LFS1, P53, Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53

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Galerie de données de validation

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  WB analysis of A431 cells using 21891-1-AP

  WB result of p53 antibody (21891-1-AP, 1:1000) with sh-control and sh-p53 transfected A431 cells.

• 

  WB analysis of A431 using 21891-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 21891-1-AP (TP53 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 21891-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21891-1-AP (TP53 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 21891-1-AP

  IP Result of anti-TP53 (IP:21891-1-AP, 3ug; Detection:21891-1-AP 1:1000) with A431 cells lysate 1650ug.

• 

  IHC staining of human prostate cancer using 21891-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21891-1-AP (TP53 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 21891-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 21891-1-AP (P53 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 21891-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 21891-1-AP (P53 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 21891-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 21891-1-AP (P53 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 21891-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 21891-1-AP (TP53 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 21891-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 21891-1-AP (TP53 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary tumor using 21891-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 21891-1-AP (TP53 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human endometrial cancer using 21891-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 21891-1-AP (TP53 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 21891-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21891-1-AP (TP53 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 21891-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 21891-1-AP (TP53 antibody) at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human endometrial cancer using 21891-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 21891-1-AP (TP53 antibody) at dilution of 1:400 (under 40x lens).

• 

  IF Staining of HepG2 using 21891-1-AP

  Immunofluorescent analysis of HepG2 cells using 21891-1-AP (TP53 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293
• Résultats positifs en IP: cellules A431
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu de cancer de l'endomètre humain, tissu de gliome humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 29 publications below
• IHC: See 13 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

21891-1-AP cible P53 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: P53 Protéine recombinante Ag16596
• Nom complet: tumor protein p53
• Masse moléculaire calculée: 393 aa, 44 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: BC003596
• Symbole du gène: TP53
• Identification du gène (NCBI): 7157
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

1.       What is p53?

P53 is a tumor suppressor gene that plays a role in maintaining genomic stability and controlling apoptosis. During the cell cycle, it can arrest cells at the G1/S checkpoint and activate DNA repair mechanisms. It is the most mutated gene in cancer. In unstressed cells, p53 usually exists at low levels in an inactive form, being bound to Mdm2.

2.       FAQs and p53

a.       I fail to detect p53 by western blotting

Basal levels of wild-type p53 in untreated cells can be low. Try to load more cell lysate and use a positive control - a lysate of cells treated with DNA-damaging agents should increase p53 levels.

b.      I fail to detect p53 in some cell lines by western blotting

Various p53 mutations are present in cancer cell types. If mutations cause truncations/deletions some monoclonal antibodies may no longer recognize mutated p53. You have more chances of detecting various p53 mutants with our polyclonal antibody.

c.       I can detect more than one band ~50 kDa size / different cell lines give bands at slightly different size

p53 is a subject of post-translational modifications (http://p53.free.fr/p53_info/p53_modifications.html) and more than one isoform may be expressed (http://p53.free.fr/p53_info/p53_isoforms.html). Also, it is possible that your cell line of interest expresses one allele with mutated p53 with altered molecular weight.

Publications

Species	Application	Title
human	WB	Cancer Cell; p53 Is a Master Regulator of Proteostasis in SMARCB1-Deficient Malignant Rhabdoid Tumors.; Authors - Alessandro Carugo ; KD Validated
human	WB,IHC,CoIP	Cancer Lett; Choline-induced SLC5A7 impairs colorectal cancer growth by stabilizing p53 protein.; Authors - Yuan Yin
mouse	WB	Oxid Med Cell Longev; PIN1 Protects Hair Cells and Auditory HEI-OC1 Cells against Senescence by Inhibiting the PI3K/Akt/mTOR Pathway.; Authors - Yanzhuo Zhang
human,mouse	WB,IHC	Mol Ther Nucleic Acids; LINC00460-miR-149-5p/miR-150-5p-Mutant p53 Feedback Loop Promotes Oxaliplatin Resistance in Colorectal Cancer.; Authors - Xiangfeng Meng
human	WB	J Nanobiotechnology; Extracellular vesicles derived from hypoxia-preconditioned olfactory mucosa mesenchymal stem cells enhance angiogenesis via miR-612; Authors - Lite Ge ; KD Validated
mouse	IHC	J Nanobiotechnology; Tryptophan-sorbitol based carbon quantum dots for theranostics against hepatocellular carcinoma.; Authors - Yang Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Y (Verified Customer) (04-01-2019)

We really like the trial size, it lets us try to test if the antibody works on our experiment.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 200

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