Anticorps Polyclonal de lapin anti-TP53BP1-Specific

TP53BP1-Specific Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20002-1-PBS

Synonymes

TP53BP1, 53BP1, FLJ41424, p202, p53 binding protein 1

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Galerie de données de validation

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  WB analysis using 20002-1-AP (same clone as 20002-1-PBS)

  HeLa lysates were subjected to SDS PAGE followed by western blot with 20002-1-AP (TP53BP1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20002-1-PBS in a different storage buffer formulation.

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  WB analysis using 20002-1-AP (same clone as 20002-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20002-1-AP (TP53BP1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20002-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 20002-1-AP (same clone as 20002-1-PBS)

  Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 20002-1-AP (TP53BP1-Specific antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20002-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 20002-1-AP (same clone as 20002-1-PBS)

  Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 20002-1-AP (TP53BP1-Specific antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20002-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 20002-1-AP (same clone as 20002-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TP53BP1-Specific antibody (20002-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 20002-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20002-1-PBS cible TP53BP1-Specific dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: tumor protein p53 binding protein 1
• Masse moléculaire calculée: 214 kDa
• Poids moléculaire observé: 450 kDa
• Numéro d’acquisition GenBank: NM_005657
• Symbole du gène: TP53BP1
• Identification du gène (NCBI): 7158
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TP53BP1, also named as p53BP1, p202, 53BP1 and FLJ41424, may have a role in checkpoint signaling during mitosis. TP53BP1 enhances TP53-mediated transcriptional activation. TP53BP1 plays a role in the response to DNA damage. This antibody is specific to TP53BP1.

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