Anticorps Polyclonal de lapin anti-hD53; TPD52L1

• Phare
• Validé par KD/KO

hD53; TPD52L1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 14732-1-AP

Synonymes

D53, hD53, hD53; TPD52L1, TDP53, TPD52L1, TPD52L2, TPD53, tumor protein D52 like 1, Tumor protein D53

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Galerie de données de validation

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  WB analysis of MCF-7 using 14732-1-AP

  WB result of hD53; TPD52L1 antibody (14732-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-hD53; TPD52L1 transfected MCF-7 cells.

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  WB analysis of MCF-7 using 14732-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14732-1-AP (hD53; TPD52L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 14732-1-AP

  IP Result of anti-hD53; TPD52L1 (IP:14732-1-AP, 3ug; Detection:14732-1-AP 1:300) with MCF-7 cells lysate 2500ug.

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  IHC staining of human prostate cancer using 14732-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14732-1-AP (hD53; TPD52L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 14732-1-AP

  Immunofluorescent analysis of MCF-7 cells using 14732-1-AP (hD53; TPD52L1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules MCF7, cellules MCF-7
• Résultats positifs en IP: cellules MCF-7
• Résultats positifs en IHC: tissu de cancer de la prostate humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

14732-1-AP cible hD53; TPD52L1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: hD53; TPD52L1 Protéine recombinante Ag6493
• Nom complet: tumor protein D52-like 1
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC002375
• Symbole du gène: TPD52L1
• Identification du gène (NCBI): 7164
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tumor protein D52-like 1 (TPD52L1) gene, also known as hD53, encodes a member of the tumor protein D52 (TPD52) family. TPD52-like proteins are coiled-coil motif-bearing proteins first identified through their expression in human breast carcinoma, which have been proposed to represent signaling intermediates and regulators of vesicle trafficking. hD53 may form homo- or heterodimers with other TPD52 family members, and it is reported to be involved in cell proliferation and calcium signaling. Four variants resulted from alternative splicing have been predicated and this antibody is expected to recognize all the variants.

Publications

Species	Application	Title
human	WB	Cell Biosci; Tumor protein D52 is upregulated in oral squamous carcinoma cells under hypoxia in a hypoxia-inducible-factor-independent manner and is involved in cell death resistance.; Authors - Yuzo Abe
human	IHC	Front Oncol; Transcriptomic Analyses of the Adenoma-Carcinoma Sequence Identify Hallmarks Associated With the Onset of Colorectal Cancer.; Authors - Qin Hong
human,mouse	WB,IHC,IP	Int J Oncol; Opposite effects of tumor protein D (TPD) 52 and TPD54 on oral squamous cell carcinoma cells.; Authors - Kosuke Kato
	WB	Biochem J; Tumor protein D52 expression is post-transcriptionally regulated by T-cell intercellular antigen (TIA) 1 and TIA-related protein via mRNA stability.; Authors - Hiromi Motohashi
		Sci Data; The Signaling Pathways Project, an integrated 'omics knowledgebase for mammalian cellular signaling pathways.; Authors - Scott A Ochsner