Anticorps Polyclonal de lapin anti-TPD52L2

• Phare
• Validé par KD/KO

TPD52L2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

8

Avis (1)

N° de cat : 11795-1-AP

Synonymes

D54, hD54, TPD52L2, TPD54, tumor protein D52 like 2, Tumor protein D54

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Galerie de données de validation

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  WB analysis using 11795-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11795-1-AP (TPD52L2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 11795-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11795-1-AP (TPD52L2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 11795-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11795-1-AP (TPD52L2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 11795-1-AP

  IP Result of anti-TPD52L2 (IP:11795-1-AP, 3ug; Detection:11795-1-AP 1:800) with HEK-293 cells lysate 2400ug.

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  IHC staining of human breast cancer using 11795-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11795-1-AP (TPD52L2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 11795-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11795-1-AP (TPD52L2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11795-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 11795-1-AP (TPD52L2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules MCF-7, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

11795-1-AP cible TPD52L2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TPD52L2 Protéine recombinante Ag2364
• Nom complet: tumor protein D52-like 2
• Masse moléculaire calculée: 206 aa, 22 kDa
• Poids moléculaire observé: 25-30 kDa
• Numéro d’acquisition GenBank: BC006804
• Symbole du gène: TPD52L2
• Identification du gène (NCBI): 7165
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tumor protein D52-like proteins (TPD52) are small coiled-coil motif bearing proteins that were first identified in breast carcinoma. Three human TPD52 members had been identified, named hD52 (TPD52), hD53 (TPD52L1), and hD54 (TPD52L2). The most important characteristic of the protein family is a highly conserved coiled-coil motif that is required for homo- and heteromeric interaction with other TPD52-like proteins. TPD52 and related proteins have been implicated in cell proliferation, apoptosis, and vesicle trafficking. TPD52L2 has five isoforms produced by alternative splicing, and its multiple sites have been identified to be phosphorylated. Interaction of TPD52L2 with MAL2, a novel member of the MAL proteolipid family, may be required for the role of TPD52L2 in vesicle transport.

Publications

Species	Application	Title
human	WB	Carcinogenesis; TPD52L2 impacts proliferation, invasiveness and apoptosis of glioblastoma cells via modulation of wnt/β-catenin/snail signaling.; Authors - Zhou Qiang ; KD Validated
human	WB	Cell Biosci; Tumor protein D52 is upregulated in oral squamous carcinoma cells under hypoxia in a hypoxia-inducible-factor-independent manner and is involved in cell death resistance.; Authors - Yuzo Abe
human	IF	J Biol Chem; Tumor protein D54 binds intracellular nanovesicles via an extended amphipathic region.; Authors - Antoine Reynaud
human,mouse	WB,IHC,IP	Int J Oncol; Opposite effects of tumor protein D (TPD) 52 and TPD54 on oral squamous cell carcinoma cells.; Authors - Kosuke Kato
mouse	WB,IHC	Biomed Res Int; Tumor Proteins D52 and D54 Have Opposite Effects on the Terminal Differentiation of Chondrocytes.; Authors - Chihiro Ito
human	WB	Neoplasma; Silencing of TPD52 inhibits proliferation, migration, invasion but induces apoptosis of pancreatic cancer cells by deactivating Akt pathway.; Authors - Z Wang ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jerome (Verified Customer) (01-05-2020)

Puncta in the cytoplasm

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: HeLa cells

TPD52L2 Antibody Immunofluorescence, validation (1:100 dilution) in HeLa cells (Cat no:11795-1-AP)