Anticorps Monoclonal anti-TPD52L2

TPD52L2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

3B3C6

N° de cat : 68077-1-PBS

Synonymes

D54, DKFZp686A1765, hD54, TPD52L2, TPD54, tumor protein D52 like 2, Tumor protein D54

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Galerie de données de validation

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  WB analysis using 68077-1-Ig (same clone as 68077-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68077-1-Ig (TPD52L2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68077-1-PBS in a different storage buffer formulation.

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  WB analysis of NCI-H1299 using 68077-1-Ig (same clone as 68077-1-PBS)

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 68077-1-Ig (TPD52L2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68077-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 68077-1-Ig (same clone as 68077-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 68077-1-Ig (TPD52L2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68077-1-PBS in a different storage buffer formulation.

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  WB analysis of Neuro-2a using 68077-1-Ig (same clone as 68077-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 68077-1-Ig (TPD52L2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68077-1-PBS in a different storage buffer formulation.

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  WB analysis of C6 using 68077-1-Ig (same clone as 68077-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 68077-1-Ig (TPD52L2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68077-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 68077-1-Ig (same clone as 68077-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68077-1-Ig (TPD52L2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68077-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 68077-1-Ig (same clone as 68077-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68077-1-Ig (TPD52L2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68077-1-PBS in a different storage buffer formulation.

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  IF Staining of HCT 116 using 68077-1-Ig (same clone as 68077-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HCT 116 cells using TPD52L2 antibody (68077-1-Ig, Clone: 3B3C6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68077-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68077-1-PBS cible TPD52L2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: tumor protein D52-like 2
• Masse moléculaire calculée: 206 aa, 22 kDa
• Poids moléculaire observé: 25-30 kDa
• Numéro d’acquisition GenBank: BC006804
• Symbole du gène: TPD52L2
• Identification du gène (NCBI): 7165
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Tumor protein D52-like proteins (TPD52) are small coiled-coil motif bearing proteins that were first identified in breast carcinoma. Three human TPD52 members had been identified, named hD52 (TPD52), hD53 (TPD52L1), and hD54 (TPD52L2). The most important characteristic of the protein family is a highly conserved coiled-coil motif that is required for homo- and heteromeric interaction with other TPD52-like proteins. TPD52 and related proteins have been implicated in cell proliferation, apoptosis, and vesicle trafficking. TPD52L2 has five isoforms produced by alternative splicing, and its multiple sites have been identified to be phosphorylated. Interaction of TPD52L2 with MAL2, a novel member of the MAL proteolipid family, may be required for the role of TPD52L2 in vesicle transport.

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