Anticorps Polyclonal de lapin anti-TPH2

TPH2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 29283-1-AP

Synonymes

EC:1.14.16.4, NTPH, TPH 2, Tryptophan 5-hydroxylase 2, Tryptophan 5-monooxygenase 2

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Galerie de données de validation

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  WB analysis using 29283-1-AP

  Rat brain tissue were subjected to SDS PAGE followed by western blot with 29283-1-AP (TPH2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 29283-1-AP

  Mouse brain tissue were subjected to SDS PAGE followed by western blot with 29283-1-AP (TPH2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 29283-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29283-1-AP (TPH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 29283-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 29283-1-AP (TPH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 29283-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 29283-1-AP (TPH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 29283-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29283-1-AP (TPH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

29283-1-AP cible TPH2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TPH2 Protéine recombinante Ag30281
• Nom complet: tryptophan hydroxylase 2
• Masse moléculaire calculée: 490 aa, 56 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC114442
• Symbole du gène: TPH2
• Identification du gène (NCBI): 121278
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TPH2 protein is a key enzyme in the synthesis of serotonin in the brain. It is mainly expressed in the brain and is responsible for converting tryptophan to 5-hydroxytryptophan, a precursor of serotonin. TPH2 plays a crucial role in regulating mood, anxiety, and stress responses. Variations in the TPH2 gene have been associated with several psychiatric disorders, including depression and bipolar disorder. Additionally, TPH2 expression can be regulated by various factors, such as hormones and environmental stimuli (PMID: 22241550, PMID: 29775696, PMID: 32119710, PMID: 40204433).

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