Anticorps Monoclonal anti-TPI1

• Phare
• Validé par KD/KO

TPI1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2E7E5

N° de cat : 67515-1-PBS

Synonymes

TIM, TPI, TPI1, Triose phosphate isomerase, Triosephosphate isomerase, triosephosphate isomerase 1

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Galerie de données de validation

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  WB analysis using 67515-1-Ig (same clone as 67515-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67515-1-Ig (TPI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67515-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67515-1-Ig (same clone as 67515-1-PBS)

  WB result of TPI1 antibody (67515-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPI1 transfected HEK-293 cells. This data was developed using the same antibody clone with 67515-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67515-1-Ig (same clone as 67515-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67515-1-Ig (TPI1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67515-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67515-1-Ig (same clone as 67515-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67515-1-Ig (TPI1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67515-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67515-1-Ig (same clone as 67515-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TPI1 antibody (67515-1-Ig, Clone: 2E7E5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67515-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67515-1-PBS cible TPI1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TPI1 Protéine recombinante Ag16653
• Nom complet: triosephosphate isomerase 1
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC007086
• Symbole du gène: TPI1
• Identification du gène (NCBI): 7167
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TPI1(Triosephosphate isomerase) is also named as TIM, TPI and belongs to the triosephosphate isomerase family. It is a key enzyme in cell metabolism that controls the glycolytic flow and energy production through the interconversion of dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde 3-phosphate (G3P)(PMID:19251756). Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency). It can exsit as a homodimer(PMID:18562316).

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