Anticorps Polyclonal de lapin anti-TPM3

• Phare
• Validé par KD/KO

TPM3 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(13)

N° de cat : 10737-1-AP

Synonymes

Gamma-tropomyosin, Tropomyosin alpha 3 chain, Tropomyosin alpha-3 chain, Tropomyosin-3, Tropomyosin-5

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Galerie de données de validation

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  WB analysis of mouse skeletal muscle using 10737-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10737-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 antibody) at dilution of 1:80000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10737-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10737-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 10737-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 10737-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 10737-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 10737-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10737-1-AP

  IP result of anti-TPM3 (IP:10737-1-AP, 4ug; Detection:10737-1-AP 1:300) with HeLa cells lysate 2000ug.

• 

  IHC staining of human colon cancer using 10737-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10737-1-AP (TPM3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10737-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10737-1-AP (TPM3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de souris, cellules HEK-293, cellules HeLa, tissu cardiaque de rat, tissu cardiaque de souris, tissu cardiaque humain, tissu de muscle squelettique de rat
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

10737-1-AP cible TPM3 dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TPM3 Protéine recombinante Ag1171
• Nom complet: tropomyosin 3
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC008407
• Symbole du gène: TPM3
• Identification du gène (NCBI): 7170
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tropomyosins are ubiquitous proteins of 35 to 45 kDa associated with the actin filaments of myofibrils and stress fibers. Tropomyosins are dimers of coiled-coil proteins that provide stability to actin filaments and regulate access of other actin-binding proteins.TPM2 subunit constitutes one part of the TPM dimer, and TPM1 or TPM3 constitutes the other part of the TPM dimer (22064019), causing an extra band of ~70kDa.

Publications

Species	Application	Title
human	WB	Bone Res; Impairment of rigidity sensing caused by mutant TP53 gain of function in osteosarcoma; Authors - Ming Luo
	IP	Nucleic Acids Res; Short DNA/RNA heteroduplex oligonucleotide interacting proteins are key regulators of target gene silencing.; Authors - Ken Asada
human	WB	Oncogene; MTBP suppresses cell migration and filopodia formation by inhibiting ACTN4.; Authors - Agarwal N N
mouse	WB,CoIP	J Am Heart Assoc; Modulation of Abnormal Vasoconstriction Through 2-Hydroxyisobutyrylation of Tropomyosin 3 Lys141: Targeting Histone Deacetylase 3 as a Key Approach; Authors - Pan-Pan Pang
mouse,human	WB	Oncol Res; LncRNA WEE2-AS1 Knockdown Inhibits the Proliferation, Migration and 3 Invasion of Glioma Cells via Regulating miR-29b-2-5p/TPM3 Axis.; Authors - Zhen Jia
	WB	J Proteome Res; Multi-Proteomic Analysis Reveals the Effect of Protein Lactylation on Matrix and Cholesterol Metabolism in Tendinopathy; Authors - Yuan Lin

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