Anticorps Monoclonal anti-TPM4

• Phare
• Validé par KD/KO

TPM4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(5)

CloneNo.

2E12D12

N° de cat : 67244-1-Ig

Synonymes

2E12D12, TM30p1, tropomyosin 4, Tropomyosin alpha 4 chain, Tropomyosin alpha-4 chain

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Galerie de données de validation

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  WB analysis using 67244-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67244-1-Ig (TPM4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67244-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67244-1-Ig (TPM4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 67244-1-Ig

  WB result of TPM4 antibody (67244-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPM4 transfected A549 cells.

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  WB analysis using 67244-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67244-1-Ig (TPM4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 67244-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67244-1-Ig (TPM4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat heart using 67244-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67244-1-Ig (TPM4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 67244-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TPM4 antibody (67244-1-Ig, Clone: 2E12D12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de rat, cellules A549, cellules HeLa, cellules HepG2, cellules HT-29, tissu cardiaque de lapin, tissu cardiaque de porc, tissu cardiaque de rat, tissu cardiaque de souris, tissu cardiaque humain, tissu de muscle squelettique de lapin, tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu cardiaque de souris, tissu cardiaque de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

67244-1-Ig cible TPM4 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TPM4 Protéine recombinante Ag6947
• Nom complet: tropomyosin 4
• Masse moléculaire calculée: 248 aa, 29 kDa
• Poids moléculaire observé: 32-35 kDa
• Numéro d’acquisition GenBank: BC037576
• Symbole du gène: TPM4
• Identification du gène (NCBI): 7171
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TPM4 is a member of tropomyosins (TPMs), a multi-gene family of actin-binding proteins present in all eukaryotic cells. In muscle, TPMs are responsible for mediating contraction via regulation of the actin-myosin interaction. As to non-muscle cells, its proposed role is to stabilize the actin filaments by modulating the interaction with proteins that are responsible for the regulation of actin dynamics. The form of TPM4 in muscle has a higher molecular weight than the form found in non-muscle cells.

Publications

Species	Application	Title
mouse	WB	CNS Neurosci Ther; Plasma-Derived Exosomal i-tRF-LeuCAA as Biomarker for Glioma Diagnosis and Promoter of Epithelial-Mesenchymal Transition via TPM4 Regulation; Authors - Hongyu Liu
human,mouse	IHC	Clin Exp Med; TPM4 influences the initiation and progression of gastric cancer by modulating ferroptosis via SCD1; Authors - Ling-Lin Zhao
human	WB	Rheumatology (Oxford); Association of Anti-TPM4 autoantibodies with vasculopathic cutaneous manifestations in juvenile dermatomyositis; Authors - Rie Karasawa
mouse	WB	PLoS One; Proteomic changes in the hippocampus of large mammals after total-body low dose radiation; Authors - Diego Iacono
human	IF	Cell Discov; TPM4 condensates glycolytic enzymes and facilitates actin reorganization under hyperosmotic stress; Authors - Wenzhong Yang

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