Anticorps Polyclonal de lapin anti-TPMT

TPMT Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 10682-1-AP

Synonymes

Thiopurine S-methyltransferase, EC:2.1.1.67

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Galerie de données de validation

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  WB analysis using 10682-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10682-1-AP (TPMT antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 10682-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10682-1-AP (TPMT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 10682-1-AP

  Immunofluorescent analysis of HEK-293 cells using 10682-1-AP (TPMT antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu rénal de souris
• Résultats positifs en IF/ICC: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below

Informations sur le produit

10682-1-AP cible TPMT dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TPMT Protéine recombinante Ag1074
• Nom complet: thiopurine S-methyltransferase
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 28-32 kDa
• Numéro d’acquisition GenBank: BC005339
• Symbole du gène: TPMT
• Identification du gène (NCBI): 7172
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The TPMT gene is localized to chromosome 6p22.3 and is approximately 34 kDa in length. It has an open reading frame (ORF) of 735 bp that encodes for a 245-amino acid peptide. The wild type TPMT allele, expressing the enzyme with normal activity. To date, there have been 30 variant TPMT alleles described (PMID: 22493988). Thiopurine compounds are commonly used in the treatment of childhood acute lymphoblastic leukemia, and as immunosuppressants following organ transplantation or for treatment of various autoimmune disorders. Thiopurine S-methyltransferase (TPMT) is required for detoxification, through S-methylation, of 6-thioguanine nucleotides (TGNs), a byproduct of thiopurine metabolism. Catalog#10682-1-AP is a rabbit polyclonal antibody raised against the full-length of human TPMT. TPMT has a predicted molecular weight of 28 kDa, however, as a result of its phosphorylation it separates on a SDS gel as a band of approximately 28-32 kDa.

Publications

Species	Application	Title
human	IHC	Front Cell Dev Biol; Predicting Panel of Metabolism and Immune-Related Genes for the Prognosis of Human Ovarian Cancer.; Authors - Lingyun Zhang
	WB	Metabolites; A Rapid and Reliable Absorbance Assay to Identify Drug-Drug Interactions with Thiopurine Drugs; Authors - Drake A Russell
sheep	WB	PLoS One; TMT-based quantitative proteomics reveals the genetic mechanisms of secondary hair follicle development in fine-wool sheep; Authors - Li-Xia Qiu

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