Anticorps Monoclonal anti-TPPII

• Phare
• Validé par KD/KO

TPPII Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

5H6F11

N° de cat : 66017-1-PBS

Synonymes

TPP 2, TPP2, TPPII, Tripeptidyl aminopeptidase, Tripeptidyl peptidase 2, tripeptidyl peptidase II

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Galerie de données de validation

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  WB analysis of Jurkat using 66017-1-Ig (same clone as 66017-1-PBS)

  WB result of TPPII antibody (66017-1-Ig, 1:4000) with si-control and si-TPPII transfected Jurkat cells. This data was developed using the same antibody clone with 66017-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66017-1-Ig (same clone as 66017-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66017-1-Ig (TPPII antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66017-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66017-1-Ig (same clone as 66017-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66017-1-Ig (TPPII antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66017-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 66017-1-Ig (same clone as 66017-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 66017-1-Ig (TPPII antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66017-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66017-1-Ig (same clone as 66017-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 66017-1-Ig(TPPII antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66017-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66017-1-Ig (same clone as 66017-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 66017-1-Ig(TPPII antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66017-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66017-1-Ig (same clone as 66017-1-PBS)

  Immunofluorescent analysis of Hela cells, using TPP2 antibody 66017-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 66017-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 66017-1-Ig (same clone as 66017-1-PBS)

  Immunofluorescent analysis of MCF-7 cells, using TPP2 antibody 66017-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 66017-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66017-1-PBS cible TPPII dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TPPII Protéine recombinante Ag5296
• Nom complet: tripeptidyl peptidase II
• Masse moléculaire calculée: 1249 aa, 138 kDa
• Poids moléculaire observé: 135 kDa
• Numéro d’acquisition GenBank: BC039905
• Symbole du gène: TPPII
• Identification du gène (NCBI): 7174
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TPPII(Tripeptidyl-peptidase 2) is an evolutionary conserved serine peptidase of the subtilisin family that removes tripeptides from the free NH2 terminus of oligopeptides. The 138-kDa TPP2 polypeptide forms large oligomeric complexes of >1,000 kDa that are detected in the cytoplasm of all cell types. In addition, a membrane associated TPP 2 isoform is present in most cells(PMID:15735022).

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