Anticorps Polyclonal de lapin anti-TPPP

TPPP Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 18742-1-AP

Synonymes

25 kDa brain specific protein, p24, p25, p25 alpha, p25alpha, TPPP, TPPP/p25, TPPP1

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Galerie de données de validation

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  WB analysis of mouse brain using 18742-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18742-1-AP (TPPP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 18742-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18742-1-AP (TPPP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 18742-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 18742-1-AP (TPPP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 18742-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 18742-1-AP (TPPP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 18742-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18742-1-AP (TPPP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 18742-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18742-1-AP (TPPP antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules MCF-7, tissu splénique de souris
• Résultats positifs en IHC: tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

18742-1-AP cible TPPP dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: tubulin polymerization promoting protein
• Masse moléculaire calculée: 219 aa, 24 kDa
• Poids moléculaire observé: 24-28 kDa
• Numéro d’acquisition GenBank: BC131506
• Symbole du gène: TPPP
• Identification du gène (NCBI): 11076
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TPPP, also named as TPPP1, P24 and P25, belongs to the TPPP family. TPPP promotes in vitro the polymerization of tubulin into double-walled tubules and polymorphic aggregates or bundled stabilized microtubules blocks. When overexpressed, TPPP inhibits mitotic spindle assembly and nuclear envelope breakdown, apparently without affecting other cellular events.

Publications

Species	Application	Title
mouse	IF	Cell; The Golgi Outpost Protein TPPP Nucleates Microtubules and Is Critical for Myelination.; Authors - Meng-Meng Fu
human	WB,IHC,IF	FASEB J; EBV-miR-BART12 accelerates migration and invasion in EBV-associated cancer cells by targeting tubulin polymerization-promoting protein 1.; Authors - Yingfen Wu
human	WB	Stem Cells Int; Enhanced Effect of IL-1β-Activated Adipose-Derived MSCs (ADMSCs) on Repair of Intestinal Ischemia-Reperfusion Injury via COX-2-PGE2 Signaling.; Authors - Liu Liu
rat	WB	J Mol Cell Cardiol; Defective autophagy triggered by arterial cyclic stretch promotes neointimal hyperplasia in vein grafts via the p62/nrf2/slc7a11 signaling pathway; Authors - Yi Chen