Anticorps Polyclonal de lapin anti-TPPP3

• Phare
• Validé par KD/KO

TPPP3 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 15057-1-AP

Synonymes

Tubulin polymerization-promoting protein family member 3, TPPP/p20, p25gamma, p20, CGI-38

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Galerie de données de validation

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  WB analysis of HEK-293 cells using 15057-1-AP

  WB result of anti-TPPP3 (15057-1-AP, 1:500) with HEK-293 cells (siRNA transfected with) by Dr. Yupeng Chen. (Negative control or TPPP3 specific siRNAs were transfected into HEK-293 cells with the final concentration of 20 nM. Forty eight hours after transfection, cells were lysed with RIPA buffer and subjected to SDS PAGE).

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  WB analysis of PC-3 using 15057-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15057-1-AP (TPPP3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 15057-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15057-1-AP (TPPP3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15057-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15057-1-AP (TPPP3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 15057-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15057-1-AP (TPPP3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules HEK-293
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

15057-1-AP cible TPPP3 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TPPP3 Protéine recombinante Ag7040
• Nom complet: tubulin polymerization-promoting protein family member 3
• Masse moléculaire calculée: 19 kDa
• Poids moléculaire observé: 19-20 kDa
• Numéro d’acquisition GenBank: BC000691
• Symbole du gène: TPPP3
• Identification du gène (NCBI): 51673
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TPPP3 (tubulin polymerization promoting protein 3), also named as TPPP/p20, is a new member of the TPPP family which binds tubulin and induces tubulin polymerization and microtubule bundling.TPPP3 has been shown to play an important role in cell proliferation and may be implicated in tumorigenesis and metastasis (19633818). TPPP3 was also identified as specific marker for the connective tissues that envelope tendons due to its distinct expression in the developing tendon sheath (19235716). This antibody detects the 19-20 kDa TPPP3 in HeLa and PC-3 cells. The non-specific bands between 40 and 60 kDa were also occasionally observed.

Publications

Species	Application	Title
mouse		Immunity; Intestinal Tuft-2 cells exert antimicrobial immunity via sensing bacterial metabolite N-undecanoylglycine.; Authors - Zhen Xiong
human	WB	Stem Cell Res Ther; Requirement of splicing factor hnRNP A2B1 for tumorigenesis of melanoma stem cells.; Authors - Mengqi Chu ; KD Validated
mouse	IF	Front Immunol; Integrated analyses of single-cell transcriptomics identify metastasis-associated myeloid subpopulations in breast cancer lung metastasis; Authors - Zhen Huang
mouse	WB	Front Dement; Quantitative proteomic analysis using a mouse model of Lewy body dementia induced by α-synuclein preformed fibrils injection; Authors - Fatih Akkentli
mouse	IF	iScience; Dynamic changes of molecular pattern and cellular subpopulation in puncture-induced tendon injury model; Authors - Zizhan Huang

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