Anticorps Recombinant de lapin anti-TPR

TPR Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

230413A2

N° de cat : 83177-5-PBS

Synonymes

Nucleoprotein TPR, 230413A2

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Galerie de données de validation

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  WB analysis using 83177-5-RR (same clone as 83177-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83177-5-RR (TPR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83177-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 83177-5-RR (same clone as 83177-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83177-5-RR (TPR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83177-5-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83177-5-RR (same clone as 83177-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TPR antibody (83177-5-RR, Clone: 230413A2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83177-5-PBS in a different storage buffer formulation.

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Informations sur le produit

83177-5-PBS cible TPR dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: translocated promoter region (to activated MET oncogene)
• Masse moléculaire calculée: 267 kDa
• Poids moléculaire observé: ~270 kDa
• Numéro d’acquisition GenBank: NM_003292
• Symbole du gène: TPR
• Identification du gène (NCBI): 7175
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Nucleoporin TPR was originally identified as the oncogenic activator of the met, raf, and trk protooncogenes. TPR is a large 270 kDa protein with a bipartite structure consisting of a ~1600-residue a-helical coiled-coil N-terminal domain and a highly acidic non-coiled ~800 amino acid carboxy terminus (PMID: 22253824). TPR is localized exclusively to intranuclear filaments associated with the nucleoplasmic side of the NPC, by directly binding to Nup153 (PMID: 12802065).

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