Anticorps Monoclonal anti-TPRKB

TPRKB Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2D5B9

N° de cat : 66402-1-PBS

Synonymes

2D5B9, CGI-121, EKC/KEOPS complex subunit TPRKB, My019, PRPK-binding protein

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Galerie de données de validation

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  WB analysis of HeLa using 66402-1-Ig (same clone as 66402-1-PBS)

  Western blot analysis of TPRKB in various cell lines using Proteintech antibody 66402-1-Ig at a dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66402-1-PBS in a different storage buffer formulation.

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  WB analysis using 66402-1-Ig (same clone as 66402-1-PBS)

  Various cells were subjected to SDS PAGE followed by western blot with 66402-1-Ig (TPRKB Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66402-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66402-1-Ig (same clone as 66402-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66402-1-Ig (TPRKB Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66402-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 66402-1-Ig (same clone as 66402-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66402-1-Ig (TPRKB antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66402-1-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 66402-1-Ig (same clone as 66402-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using TPRKB antibody (66402-1-Ig, Clone: 2D5B9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66402-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66402-1-PBS cible TPRKB dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TPRKB Protéine recombinante Ag18770
• Nom complet: TP53RK binding protein
• Masse moléculaire calculée: 175 aa, 20 kDa
• Poids moléculaire observé: 18-20 kDa
• Numéro d’acquisition GenBank: BC029492
• Symbole du gène: TPRKB
• Identification du gène (NCBI): 51002
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TPRKB, also named as CGI 121, belongs to the CGI121/TPRKB family. It interacts with TP53RK/PRPK and is widely expressed in all tissues.

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