Anticorps Monoclonal anti-TPSAB1

TPSAB1 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(3)

CloneNo.

6A10G1

Avis (1)

N° de cat : 66174-1-Ig

Synonymes

6A10G1, EC:3.4.21.59, MCP7, TPS1, TPS2

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Galerie de données de validation

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  WB analysis of human kidney using 66174-1-Ig

  human kidney tissue were subjected to SDS PAGE followed by western blot with 66174-1-Ig (TPSAB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 66174-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66174-1-Ig (TPSAB1 antibody) at dilution of 1:5000 (under 10x lens) microwave oven heating mediated antigen retrieved with .

• 

  IHC staining of human tonsillitis using 66174-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66174-1-Ig (TPSAB1 antibody) at dilution of 1:5000 (under 40x lens) microwave oven heating mediated antigen retrieved with .

• 

  IHC staining of human tonsillitis using 66174-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66174-1-Ig (TPSAB1 antibody) at dilution of 1:20000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu rénal humain
• Résultats positifs en IHC: tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

66174-1-Ig cible TPSAB1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TPSAB1 Protéine recombinante Ag4167
• Nom complet: tryptase alpha/beta 1
• Masse moléculaire calculée: 275 aa, 31 kDa
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: BC028059
• Symbole du gène: TPSAB1
• Identification du gène (NCBI): 7177
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TPSAB1(Tryptase alpha/beta-1) is also named as TPS1, TPS2, TPSB1 and belongs to the peptidase S1 family. It is the major neutral protease present in mast cells and secreted upon the coupled activation-degranulation response of this cell type. TPSAB1 has 2 isoforms produced by alternative splicing with the molecular weight of 31 kDa and 29 kDa. This full length protein has two glycosylation sites.

Publications

Species	Application	Title
human	IHC	Oncogene; Mast cells inhibit colorectal cancer development by inducing ER stress through secreting Cystatin C; Authors - Feifei Song
	WB	Int Immunopharmacol; Activation of aryl hydrocarbon receptor ameliorates degranulation of LL-37 induced mast cells in rosacea through enhancing autophagy; Authors - Shuyan Chen
Human		Mol Psychiatry; Molecular profiling of the hippocampus of children with autism spectrum disorder; Authors - Lindsay E Rexrode

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Santhosh (Verified Customer) (04-15-2019)

Human FFPE tonsilitis tissue. HIER with citrate buffer

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:200

TPSAB1 Antibody Immunohistochemistry, validation (1:200 dilution) in (Cat no:66174-1-Ig)

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