Anticorps Monoclonal anti-TPT1

• Phare
• Validé par KD/KO

TPT1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1G6A10

N° de cat : 66713-1-PBS

Synonymes

TCTP, p23, HRF, Histamine-releasing factor, Fortilin

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Galerie de données de validation

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  WB analysis using 66713-1-Ig (same clone as 66713-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66713-1-Ig (TPT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66713-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66713-1-Ig (same clone as 66713-1-PBS)

  WB result of TPT1 antibody (66713-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPT1 transfected MCF-7 cells. This data was developed using the same antibody clone with 66713-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66713-1-Ig (same clone as 66713-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66713-1-Ig (TPT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66713-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 66713-1-Ig (same clone as 66713-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66713-1-Ig (TPT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66713-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66713-1-Ig (same clone as 66713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66713-1-Ig (TPT1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66713-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66713-1-Ig (same clone as 66713-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66713-1-Ig (TPT1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66713-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 66713-1-Ig (same clone as 66713-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 66713-1-Ig (TPT1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66713-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 66713-1-Ig (same clone as 66713-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using TPT1 antibody (66713-1-Ig, Clone: 1G6A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66713-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66713-1-PBS cible TPT1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TPT1 Protéine recombinante Ag27651
• Nom complet: tumor protein, translationally-controlled 1
• Masse moléculaire calculée: 20 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC003352
• Symbole du gène: TPT1
• Identification du gène (NCBI): 7178
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The translationally controlled tumor protein (TPT1, also known as TCTP) is a highly conserved, abundantly expressed protein associated with cell growth and apoptosis regulation. It is overexpressed in most tumor cells and its down-regulation decreases the viability of those cells. This antibody detected 22 kDa TPT1 in lysates.

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