Anticorps Monoclonal anti-TPX2

TPX2 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

2E10C1

N° de cat : 68712-1-Ig

Synonymes

2E10C1, C20orf1, C20orf2, Differentially expressed in cancerous and non-cancerous lung cells 2, DIL 2

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Galerie de données de validation

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  WB analysis using 68712-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68712-1-Ig (TPX2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 68712-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68712-1-Ig (TPX2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 68712-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 68712-1-Ig (TPX2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 68712-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 68712-1-Ig (TPX2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 68712-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using TPX2 antibody (68712-1-Ig, Clone: 2E10C1 ) at dilution of 1:4000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

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  IF Staining of U2OS using 68712-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using TPX2 antibody (68712-1-Ig, Clone: 2E10C1 ) at dilution of 1:4000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules MCF-7, cellules NIH/3T3, cellules U2OS
• Résultats positifs en IF/ICC: cellules U2OS, cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68712-1-Ig cible TPX2 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TPX2 Protéine recombinante Ag33683
• Nom complet: TPX2, microtubule-associated, homolog (Xenopus laevis)
• Masse moléculaire calculée: 747 aa, 86 kDa
• Poids moléculaire observé: 98 kDa
• Numéro d’acquisition GenBank: BC020207
• Symbole du gène: TPX2
• Identification du gène (NCBI): 22974
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TPX2, also known as DIL2, p10, is a microtubule organization- and cell cycle-associated protein. In the early stage of mitosis, TPX2 is released in a RanGTP-dependent manner and plays a significant role in mitotic spindle formation and subsequent proper segregation of chromosomes during cell division. Furthermore, during interphase, TPX2 is involved in DNA damage response by regulation of γ-H2AX signals(PMID: 25914189). Aberrant expression of TPX2 leads to improper spindle assembly and chromosomal instability. Overexpressed in cancers, TPX2 is being established as the marker for the diagnosis and prognosis of malignancies(PMID: 24556998).

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