Anticorps Polyclonal de lapin anti-TRADD

• Phare
• Validé par KD/KO

TRADD Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(10)

N° de cat : 15468-1-AP

Synonymes

CD120a, TNFR1-associated DEATH domain protein, TNFRSF1A, TNFRSF1A-associated via death domain, Tumor necrosis factor receptor type 1-associated DEATH domain protein

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Galerie de données de validation

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  WB analysis using 15468-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15468-1-AP (TRADD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 15468-1-AP

  WB result of TRADD antibody (15468-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRADD transfected A549 cells.

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  WB analysis using 15468-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15468-1-AP (TRADD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 15468-1-AP

  IP result of anti-TRADD (IP:15468-1-AP, 4ug; Detection:15468-1-AP 1:500) with Jurkat cells lysate 4000 ug.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules HL-60, cellules Jurkat, cellules MCF-7
• Résultats positifs en IP: cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

15468-1-AP cible TRADD dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRADD Protéine recombinante Ag7768
• Nom complet: TNFRSF1A-associated via death domain
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC004491
• Symbole du gène: TRADD
• Identification du gène (NCBI): 8717
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Cell Res; The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8.; Authors - Jia-Yuan Zhang
human	WB	Free Radic Biol Med; TRAF2 inhibits senescence in hepatocellular carcinoma cells via regulating the ROMO1/ NAD+/SIRT3/SOD2 axis; Authors - Jiping Yao
mouse	WB,IP,IF	Free Radic Biol Med; Kv7.4 channel is a key regulator of vascular inflammation and remodeling in neointimal hyperplasia and abdominal aortic aneurysms.; Authors - Xi-Zhenzi Fan
mouse	WB	Int J Biol Sci; Mannan-Binding Lectin Deficiency Limits Inflammation-induced Myeloid-Derived Suppressor Cells Expansion via Modulating Tumor Necrosis Factor Alpha-triggered Apoptosis.; Authors - Lijun Dong
mouse	WB	Mol Med; Knockdown of circROBO2 attenuates acute myocardial infarction through regulating the miR-1184/TRADD axis.; Authors - Tian-Ping Chen
mouse	WB	Arch Dermatol Res; PeiTuQingXin formula alleviated atopic dermatitis symptoms via inhibiting TRADD/TRAF2/RIP1 complex mediated NF-κB signaling pathway activation; Authors - Zixuan Cheng

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