Anticorps Polyclonal de lapin anti-TRAF3

• Phare
• Validé par KD/KO

TRAF3 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IP, IF, ELISA

Conjugaison

Non conjugué

Publications

18

N° de cat : 18099-1-AP

Synonymes

CAP 1, CAP1, CD40 binding protein, CD40bp, CRAF1, LAP1, LMP1 associated protein 1, TRAF3

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Galerie de données de validation

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  WB analysis using 18099-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18099-1-AP (TRAF3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 18099-1-AP

  WB result of TRAF3 antibody (18099-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRAF3 transfected HEK-293 cells.

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  WB analysis using 18099-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18099-1-AP (TRAF3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of multi-cells using 18099-1-AP

  WB results of 18099-1-aP (anti TRAF3).

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  WB analysis of HEK-293 using 18099-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18099-1-AP (TRAF3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 18099-1-AP

  IP result of anti-TRAF3 (IP:18099-1-AP, 4ug; Detection:18099-1-AP 1:1000) with Raji cells lysate 3160 ug.

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules HEK-293, cellules HeLa, cellules NIH/3T3, cellules Raji, multi-cellules
• Résultats positifs en IP: cellules Raji,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 17 publications below
• IF: See 1 publications below

Informations sur le produit

18099-1-AP cible TRAF3 dans les applications de WB, IP, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRAF3 Protéine recombinante Ag12185
• Nom complet: TNF receptor-associated factor 3
• Masse moléculaire calculée: 568 aa, 64 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: BC075087
• Symbole du gène: TRAF3
• Identification du gène (NCBI): 7187
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TNF receptor-associated factor 3 (TRAF3) has many alternative names including CAP-1, CD40 receptor-associated factor 1(CRAF1), CD40-binding protein(CD40BP) and LMP1-associated protein 1 (LAP1). TRAF3 regulates pathways leading to the activation of NF-kappa-B and MAP kinases, and plays a central role in the regulation of innate immune response. It modulates signaling pathways leading to the production of cytokines and interferon. As an essential constituent of several E3 ubiquitin-protein ligase complexes, TRAF3 promote 'Lys-63'-linked ubiquitination of target proteins. Moreover, TRAF3 undergoes both 'Lys-48'-linked and 'Lys-63'-linked ubiquitination in response to various stimuli and is deubiquitinated by OTUB1, OTUB2 and OTUD5. Several alternatively spliced transcript variants encoding distinct isoforms (64 kDa and 55 kDa) have been reported.

Publications

Species	Application	Title
human	WB	Signal Transduct Target Ther; TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion; Authors - Wenwen Zheng ; KD Validated
mouse	WB	J Exp Med; TRIM24 facilitates antiviral immunity through mediating K63-linked TRAF3 ubiquitination.; Authors - Qingchen Zhu
pig	WB	Nucleic Acids Res; The N6-methyladenosine RNA-binding protein YTHDF1 modulates the translation of TRAF6 to mediate the intestinal immune response.; Authors - Xin Zong
human	WB	EMBO J; Dimer-specific immunoprecipitation of active caspase-2 identifies TRAF proteins as novel activators.; Authors - Alexander C Robeson ; KD Validated
human	WB	Front Immunol; The C-Terminal Effector Domain of Non-Structural Protein 1 of Influenza A Virus Blocks IFN-β Production by Targeting TNF Receptor-Associated Factor 3.; Authors - Wei Qian
human	WB	Cancer Lett; Up-regulation of OLR1 expression by TBC1D3 through activation of TNFα/NF-κB pathway promotes the migration of human breast cancer cells.; Authors - Bei Wang

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