Anticorps Polyclonal de lapin anti-TRAF3IP3

TRAF3IP3 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 18110-1-AP

Synonymes

T3JAM, TRAF3 interacting protein 3, TRAF3IP3

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Galerie de données de validation

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  WB analysis of Jurkat using 18110-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18110-1-AP (TRAF3IP3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 18110-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18110-1-AP (TRAF3IP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 18110-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18110-1-AP (TRAF3IP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 18110-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18110-1-AP (TRAF3IP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 18110-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18110-1-AP (TRAF3IP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, tissu de cancer du côlon humain, tissu de thymus de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 2 publications below

Informations sur le produit

18110-1-AP cible TRAF3IP3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRAF3IP3 Protéine recombinante Ag12559
• Nom complet: TRAF3 interacting protein 3
• Masse moléculaire calculée: 353aa,41 kDa; 551aa,64 kDa
• Poids moléculaire observé: 64 kDa, 47 kDa, 41 kDa
• Numéro d’acquisition GenBank: BC110302
• Symbole du gène: TRAF3IP3
• Identification du gène (NCBI): 80342
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRAF3 interacting protein 3 (TRAF3IP3) is an essential adapter protein containing a coiled-coil domain that affects both innate and adaptive immunity. It plays a crucial role in the regulation of thymocyte development (PMID:26195727), and it also regulates T-cell stability and function by recruiting the serine-threonine phosphatase catalytic subunit (PPP2CA) to the lysosome, thereby facilitating the interaction of PP2Ac with the mTORC1 component RPTOR and restricting glycolytic metabolism (PMID:30115741). TRAF3IP3 responses to viral infection, facilitates the recruitment of TRAF3 to MAVS within mitochondria leading to IRF3 activation and interferon production (PMID:31390091). TRAF3IP3 has 3 isoforms produced by alternative splicing with the molecular weight of 64 kDa, 47 kDa and 41 kDa.

Publications

Species	Application	Title
human	IHC	Braz J Med Biol Res; SNORA5A regulates tumor-associated macrophage M1/M2 phenotypes via TRAF3IP3 in breast cancer; Authors - Yiqi Zhang
human	IHC	Adv Sci (Weinh); TRAF3IP3 Induces ER Stress-Mediated Apoptosis with Protective Autophagy to Inhibit Lung Adenocarcinoma Proliferation; Authors - Guang Zhao

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