Anticorps Polyclonal de lapin anti-TRAF3IP3

TRAF3IP3 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 18110-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of Jurkat using 18110-1-AP (same clone as 18110-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18110-1-AP (TRAF3IP3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18110-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse thymus using 18110-1-AP (same clone as 18110-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18110-1-AP (TRAF3IP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18110-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 18110-1-AP (same clone as 18110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18110-1-AP (TRAF3IP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18110-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 18110-1-AP (same clone as 18110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18110-1-AP (TRAF3IP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18110-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 18110-1-AP (same clone as 18110-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18110-1-AP (TRAF3IP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18110-1-PBS in a different storage buffer formulation.

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Informations sur le produit

18110-1-PBS cible TRAF3IP3 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRAF3IP3 Protéine recombinante Ag12559
• Nom complet: TRAF3 interacting protein 3
• Masse moléculaire calculée: 353aa,41 kDa; 551aa,64 kDa
• Poids moléculaire observé: 64 kDa, 47 kDa, 41 kDa
• Numéro d’acquisition GenBank: BC110302
• Symbole du gène: TRAF3IP3
• Identification du gène (NCBI): 80342
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TRAF3 interacting protein 3 (TRAF3IP3) is an essential adapter protein containing a coiled-coil domain that affects both innate and adaptive immunity. It plays a crucial role in the regulation of thymocyte development (PMID:26195727), and it also regulates T-cell stability and function by recruiting the serine-threonine phosphatase catalytic subunit (PPP2CA) to the lysosome, thereby facilitating the interaction of PP2Ac with the mTORC1 component RPTOR and restricting glycolytic metabolism (PMID:30115741). TRAF3IP3 responses to viral infection, facilitates the recruitment of TRAF3 to MAVS within mitochondria leading to IRF3 activation and interferon production (PMID:31390091). TRAF3IP3 has 3 isoforms produced by alternative splicing with the molecular weight of 64 kDa, 47 kDa and 41 kDa.

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