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Anticorps Monoclonal anti-TRAF5

TRAF5 Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B1A7

N° de cat : 66991-1-PBS

Synonymes

1B1A7, RING finger protein 84, RNF84, TNF receptor associated factor 5, TNF receptor-associated factor 5

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of HeLa cells using TRAF5 Monoclonal antibody (66991-1-Ig)

    WB analysis of HeLa using 66991-1-Ig (same clone as 66991-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66991-1-Ig (TRAF5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66991-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using TRAF5 Monoclonal antibody (66991-1-Ig)

    WB analysis of PC-3 using 66991-1-Ig (same clone as 66991-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 66991-1-Ig (TRAF5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66991-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of LNCaP cells using TRAF5 Monoclonal antibody (66991-1-Ig)

    WB analysis of LNCaP using 66991-1-Ig (same clone as 66991-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 66991-1-Ig (TRAF5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66991-1-PBS in a different storage buffer formulation.

Informations sur le produit

66991-1-PBS cible TRAF5 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène TRAF5 Protéine recombinante Ag26070
Nom complet TNF receptor-associated factor 5
Masse moléculaire calculée 557 aa, 64 kDa
Poids moléculaire observé 64 kDa
Numéro d’acquisition GenBankBC029600
Symbole du gène TRAF5
Identification du gène (NCBI) 7188
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TRAF5 (also known as TNF receptor-associated factor 5, Ring finger protein 84, RNF8, MGC:39780) is a member of the tumor necrosis factor receptor-associated (TRAF) family of proteins that are characterized by the presence of a meprin and TRAF homology (MATH) domain, a RING-type zinc finger domain, and two TRAF-type zinc finger domains (https://www.uniprot.org/uniprot/O00463). TRAF5 is a scaffold protein that associates with and mediates signal transduction by cell surface receptors belonging to the tumor necrosis factor (TNF) superfamily, including CD27, CD30, CD40, and lymphotoxin-β receptor, positively regulating activation of the canonical NF-κB pathway and c-Jun NH2-terminal kinase (JNK) activation by these receptors (PMIDs: 8999898, 9582383, 8790348, 8663299). The RING-type zinc finger domain of TRAF5 exhibits ubiquitin protein ligase activity and is responsible for Lys-63-linked polyubiquitination of protein targets (PMIDs:23758787, 29176576). This domain has been demonstrated to be required for TRAF5-mediated activation of NF-κB (PMID:8663299).

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