Anticorps Recombinant de lapin anti-DcR2

DcR2 Recombinant Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

242916C11

N° de cat : 85425-2-PBS

Synonymes

CD264, Decoy receptor 2, TNF-related apoptosis-inducing ligand receptor 4, TNFRSF10D, TRAIL R4

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Galerie de données de validation

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  WB analysis using 85425-2-RR (same clone as 85425-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85425-2-RR (DcR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85425-2-PBS in a different storage buffer formulation.

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  WB analysis using 85425-2-RR (same clone as 85425-2-PBS)

  Human kidney lysate was subjected to SDS PAGE followed by western blot with 85425-2-RR (DcR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85425-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01996-1

  Sandwich ELISA standard curve of MP01996-1, Human DcR2 Recombinant Matched Antibody Pair - PBS only. 85425-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg3487. 85425-2-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL

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  Sample test of MP01996-1

  The mean DcR2 concentration was determined to be 8.72 ng/mL in HCT116 cell extract based on a 1.4 mg/mL extract load.

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  IHC staining of mouse liver using 85425-2-RR (same clone as 85425-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85425-2-RR (DcR2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85425-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 85425-2-RR (same clone as 85425-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85425-2-RR (DcR2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85425-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01996-1

  Cytometric bead array standard curve of MP01996-1, DcR2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85425-1-PBS. Detection antibody: 85425-2-PBS. Standard: Eg3487. Range: 0.781-100 ng/mL.

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Informations sur le produit

85425-2-PBS cible DcR2 dans les applications de WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DcR2 Protéine recombinante Eg3487
• Nom complet: tumor necrosis factor receptor superfamily, member 10d, decoy with truncated death domain
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: NM_003840.5
• Symbole du gène: DcR2
• Identification du gène (NCBI): 8793
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Decoy receptor 2 (DcR2) is a member of the tumor necrosis factor receptor (TNFR) superfamily. It functions as a decoy receptor for TNF-related apoptosis-inducing ligand (TRAIL), thereby inhibiting TRAIL-mediated apoptosis (PMID: 15538968; 9537512). It has been considered a tentative marker of cellular senescence (PMID: 16079833; 22751116). Additionally, DCR2 has been implicated in the regulation of renal fibrosis and cell senescence, contributing to the pathogenesis of diabetic nephropathy (PMID: 35661704).

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