Anticorps Polyclonal de lapin anti-TRAIP

• Phare
• Validé par KD/KO

TRAIP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(9)

N° de cat : 10332-1-AP

Synonymes

TRIP, EC:2.3.2.27, RING finger protein 206, RNF206, TRAF interacting protein

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Galerie de données de validation

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  WB analysis of mouse brain using 10332-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10332-1-AP (TRAIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse placenta using 10332-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 10332-1-AP (TRAIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10332-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10332-1-AP (TRAIP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 10332-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10332-1-AP (TRAIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 10332-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10332-1-AP (TRAIP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10332-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10332-1-AP (TRAIP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 10332-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10332-1-AP (TRAIP Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 10332-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10332-1-AP (TRAIP Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 10332-1-AP

  Immunofluorescent analysis of HepG2 cells using 10332-1-AP (TRAIP antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules A549, cellules HepG2, tissu cérébral humain, tissu placentaire de souris, tissu placentaire humain
• Résultats positifs en IHC: tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 8 publications below
• IHC: See 5 publications below
• IP: See 1 publications below

Informations sur le produit

10332-1-AP cible TRAIP dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRAIP Protéine recombinante Ag0491
• Nom complet: TRAF interacting protein
• Masse moléculaire calculée: 53 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: BC000310
• Symbole du gène: TRAIP
• Identification du gène (NCBI): 10293
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

E3 ubiquitin-protein ligase TRAIP (TRAIP) is also named RING finger protein 206 (RNF206) and TRAF-interacting protein (TRIP). TRAIP, an E3 ligase containing a RING domain, has emerged as a significant contributor to maintaining genome integrity and TRAIP inhibits proliferation and migration of BLCA cells (PMID: 38123820). TRAIP helps replisomes overcome DNA interstrand crosslinks and DNA-protein crosslinks, whereas in mitosis it triggers disassembly of all replisomes that remain on chromatin (PMID: 33317933, PMID: 30842657).

Publications

Species	Application	Title
human	WB	Int J Biol Sci; ESRG is critical to maintain the cell survival and self-renewal/pluripotency of hPSCs by collaborating with MCM2 to suppress p53 pathway; Authors - Shasha Li
mouse	WB	Sci Rep; Phycocyanin attenuates pulmonary fibrosis via the TLR2-MyD88-NF-κB signaling pathway.; Authors - Chengcheng Li
human	WB,IHC	Biomed Pharmacother; TRAIP promotes malignant behaviors and correlates with poor prognosis in liver cancer.; Authors - Zhenya Guo ; KD Validated
mouse,human	WB,IHC	Int Urol Nephrol; TRAIP suppressed apoptosis and cell cycle to promote prostate cancer proliferation via TRAF2-PI3K-AKT pathway activation; Authors - Yuan Gu ; KD Validated
human	WB,IP,IHC	Cell Death Dis; TRAIP modulates the IGFBP3/AKT pathway to enhance the invasion and proliferation of osteosarcoma by promoting KANK1 degradation.; Authors - Mi Li ; KD Validated
human	WB,IHC	Cancer Gene Ther; Silencing TRAIP suppresses cell proliferation and migration/invasion of triple negative breast cancer via RB-E2F signaling and EMT; Authors - Yan Zheng ; KD Validated

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