Anticorps Monoclonal anti-TRAPA/SSR1

TRAPA/SSR1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F2D7

N° de cat : 67925-1-PBS

Synonymes

DKFZp781N23103, FLJ14232, FLJ22100, FLJ23034, FLJ78242, FLJ93042, SSR alpha, SSR1, TRAP alpha, TRAPA

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Galerie de données de validation

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  WB analysis using 67925-1-Ig (same clone as 67925-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67925-1-Ig (TRAPA/SSR1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67925-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 67925-1-Ig (same clone as 67925-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67925-1-Ig (TRAPA/SSR1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67925-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 67925-1-Ig (same clone as 67925-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67925-1-Ig (TRAPA/SSR1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67925-1-PBS in a different storage buffer formulation.

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  IF Staining of human ovary tumor using 67925-1-Ig (same clone as 67925-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using TRAPA/SSR1 antibody (67925-1-Ig, Clone: 1F2D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67925-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67925-1-PBS cible TRAPA/SSR1 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TRAPA/SSR1 Protéine recombinante Ag30832
• Nom complet: signal sequence receptor, alpha
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC007710
• Symbole du gène: TRAPA
• Identification du gène (NCBI): 6745
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TRAPA (translocon-associated protein subunit alpha), also known as SSR1 (signal sequence receptor subunit alpha), is a 34-kDa single-spanning membrane glycoprotein of the endoplasmic reticulum (ER) (PMID: 8050590). It is a part of the tetrameric TRAP complex (TRAP-alpha, TRAP-beta, TRAP-delta and TRAP-gamma) which functions in regulating the retention of ER resident proteins (PMID: 7916687). The TRAP complex is also involved in endoplasmic reticulum-associated degradation (ERAD) (PMID: 17380188).

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