Anticorps Polyclonal de lapin anti-TRAPPC12

• Phare
• Validé par KD/KO

TRAPPC12 Polyclonal Antibody for WB, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 24555-1-PBS

Synonymes

TTC15, CGI 87, CGI-87, TPR repeat protein 15, Trafficking of membranes and mitosis

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Galerie de données de validation

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  WB analysis using 24555-1-AP (same clone as 24555-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 24555-1-AP (TRAPPC12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24555-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 24555-1-AP (same clone as 24555-1-PBS)

  WB result of TRAPPC12 antibody (24555-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRAPPC12 transfected K-562 cells. This data was developed using the same antibody clone with 24555-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 24555-1-AP (same clone as 24555-1-PBS)

  IP result of anti-TRAPPC12 (IP:24555-1-AP, 4ug; Detection:24555-1-AP 1:500) with K-562 cells lysate 1720 ug. This data was developed using the same antibody clone with 24555-1-PBS in a different storage buffer formulation.

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Informations sur le produit

24555-1-PBS cible TRAPPC12 dans les applications de WB, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRAPPC12 Protéine recombinante Ag18332
• Nom complet: tetratricopeptide repeat domain 15
• Masse moléculaire calculée: 735 aa, 79 kDa
• Poids moléculaire observé: 98 kDa
• Numéro d’acquisition GenBank: BC014164
• Symbole du gène: TRAPPC12
• Identification du gène (NCBI): 51112
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TRAPPC12, also called CGI-87, TTC15 and TRAMM, has been recently identified as subunit of TRAPP complex in two independent proteomic studies. It was suggested that TRAPPC12 was a subunit of TRAPPIII. TRAPPC12 and TECPR1 acted at distinct steps in autophagy and depletion of TRAPPC12 and/or TECPR1 increased in the number of autophagosomes and increase autophagic flux. The observed molecular weight of TRAPPC12 is 98 kDa, which is consistent with what has been described in the literature (PMID: 28240221, PMID: 38439956).

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