Anticorps Polyclonal de lapin anti-TRAPPC2

TRAPPC2 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 12484-1-AP

Synonymes

hYP38334, MBP 1 interacting protein 2A, MIP 2A, SEDL, Sedlin, SEDLP, SEDT, TRAPPC2, TRAPPC2P1, TRS20, ZNF547L

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Galerie de données de validation

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  WB analysis of human brain using 12484-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12484-1-AP (TRAPPC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 12484-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12484-1-AP (TRAPPC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 12484-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12484-1-AP (TRAPPC2 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis of human lung using 12484-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12484-1-AP (TRAPPC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12484-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12484-1-AP (TRAPPC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 12484-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12484-1-AP (TRAPPC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of PC-3 using 12484-1-AP

  IP Result of anti-TRAPPC2 (IP:12484-1-AP, 3ug; Detection:12484-1-AP 1:500) with PC-3 cells lysate 2800ug.

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, cellules PC-3, tissu cérébral de souris, tissu de thymus de souris, tissu hépatique humain, tissu pulmonaire humain
• Résultats positifs en IP: cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

12484-1-AP cible TRAPPC2 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRAPPC2 Protéine recombinante Ag3201
• Nom complet: trafficking protein particle complex 2
• Masse moléculaire calculée: 140 aa, 16 kDa
• Poids moléculaire observé: 16 kDa
• Numéro d’acquisition GenBank: BC016915
• Symbole du gène: TRAPPC2
• Identification du gène (NCBI): 6399
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRAPPC2 is one component of the TRAPP multisubunit tethering complex involved in intracellular vesicle trafficking [PMID:21525244]. It acts as an adaptor for the TRAPP complex in mammalian cells, mediating interactions with both TRAPPC9 and TRAPPC8. It prevents transcriptional repression and induction of cell death by ENO1. Also it plays a role in vesicular transport from endoplasmic reticulum to Golgi.

Publications

Species	Application	Title
human	WB	Proc Natl Acad Sci U S A; Primary cilia membrane assembly is initiated by Rab11 and transport protein particle II (TRAPPII) complex-dependent trafficking of Rabin8 to the centrosome.; Authors - Westlake Christopher J CJ
human	WB	J Biol Chem; The C7orf43/TRAPPC14 component links the TRAPPII complex to RABIN8 for preciliary vesicle tethering at the mother centriole during ciliogenesis.; Authors - Adrian Cuenca